Mouse IL-10 ELISA Kit | IL10 elisa kit

Mouse IL-10 ELISA Kit

Cat. Num. AAA824871

• Gene Names: Il10; CSIF; Il-10
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: IL-10; Mouse IL-10 ELISA Kit; Interleukin-10; Cytokine synthesis inhibitory factor; CSIF; IL10 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse IL-10 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IL-10 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 178

• Applicable Applications for IL10 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Sensitivity: <7 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL10 elisa kit

Principle of the Assay: The Mouse IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-10 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.!!Background/Introduction: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.

NCBI and Uniprot Product Information

• NCBI GI #: 6754318
• NCBI GeneID: 16153
• NCBI Accession #: NP_034678.1
• NCBI GenBank Nucleotide #: NM_010548.2
• UniProt Accession #: P18893; Q0VBJ1
• Molecular Weight: 20,641 Da
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: Il10
• NCBI Official Synonym Symbols: CSIF; Il-10
• NCBI Protein Information: interleukin-10; cytokine synthesis inhibitory factor
• UniProt Protein Name: Interleukin-10
• Protein Family: Interleukin
• UniProt Gene Name: Il10
• UniProt Synonym Gene Names: Il-10; IL-10; CSIF
• UniProt Entry Name: IL10_MOUSE

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Secreted, signal peptide; Cytokine; Secreted; Motility/polarity/chemotaxis

Cellular Component: extracellular space; extracellular region

Molecular Function: cytokine activity; interleukin-10 receptor binding

Biological Process: negative regulation of chronic inflammatory response to antigenic stimulus; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; positive regulation of MHC class II biosynthetic process; response to molecule of bacterial origin; negative regulation of interferon-gamma production; negative regulation of interleukin-6 production; negative regulation of tumor necrosis factor biosynthetic process; inflammatory response; negative regulation of interleukin-12 production; positive regulation of B cell apoptosis; negative regulation of nitric oxide biosynthetic process; negative regulation of myeloid dendritic cell activation; negative regulation of tumor necrosis factor production; negative regulation of cytokine secretion during immune response; regulation of gene expression; negative regulation of inflammatory response; negative regulation of cytokine production; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of transcription factor activity; receptor biosynthetic process; regulation of sensory perception of pain; positive regulation of cytokine secretion; negative regulation of apoptosis