Human IL-10 ELISA Kit | IL10 elisa kit

Human IL-10 PicoKine ELISA Kit

Cat. Num. AAA175968

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Reactivity: Human
• Synonyms: IL-10; Human IL-10 PicoKine ELISA Kit; Interleukin-10; interleukin 10; CSIF; Cytokine synthesis inhibitory factor; GVHDS; IL 10; IL10; IL10_HUMAN; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; T cell growth inhibitory factor; TGIF; IL10 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human HSPG2
• Sequence Length: 178

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate)
• Detection Range: 0.624ng/ml-40ng/ml
• Sensitivity: <0.05ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL10 elisa kit

Principle of the Assay: The Picokine Human HSPG2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human HSPG2 with a 96-well strip plate that is pre-coated with antibody specific for HSPG2. The detection antibody is a biotinylated antibody specific for HSPG2. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human HSPG2 with immunogen: Expression system for standard: NSO, Immunogen sequence: E3687-S4391. The kit is analytically validated with ready to use reagents. To measure Human HSPG2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human HSPG2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human HSPG2 in the sample!!Background/Introduction: Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.

NCBI and Uniprot Product Information

• NCBI GI #: 124292
• NCBI GeneID: 3586
• UniProt Accession #: P22301
• Molecular Weight: 20,517 Da
• NCBI Official Full Name: Interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor
• UniProt Protein Name: Interleukin-10
• Protein Family: Interleukin
• UniProt Gene Name: IL10
• UniProt Synonym Gene Names: IL-10; CSIF
• UniProt Entry Name: IL10_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Motility/polarity/chemotaxis; Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; interleukin-10 receptor binding

Biological Process: B cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; regulation of isotype switching; positive regulation of transcription, DNA-dependent; leukocyte chemotaxis; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; negative regulation of T cell proliferation; cytoplasmic sequestering of NF-kappaB; cell-cell signaling; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; response to molecule of bacterial origin; negative regulation of interleukin-6 production; hemopoiesis; negative regulation of tumor necrosis factor biosynthetic process; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; positive regulation of B cell apoptosis; T-helper 2 type immune response; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; receptor biosynthetic process; response to activity; regulation of sensory perception of pain; negative regulation of apoptosis; positive regulation of cytokine secretion

Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To