IGF-2 elisa kit

Rabbit IGF-2 PicoKine ELISA Kit

Cat. Num. AAA1751041

• Gene Names: IGF2; GRDF; IGF-II; PP9974; C11orf43
• Reactivity: Rabbit; There is no detectable cross-reactivity with IGF-1.
• Applications: ELISA
• Synonyms: IGF-2; Rabbit IGF-2 PicoKine ELISA Kit; Insulin-like growth factor II; IGF-II; Somatomedin-A; T3M-11-derived growth factor; Insulin-like growth factor II Ala-25 Del; Preptin; IGF2; PP1446; IGF-2 elisa kit

• Reactivity: Rabbit; There is no detectable cross-reactivity with IGF-1.
• Specificity: Natural and recombinant Rabbit Rabbit IGF-2
• Sequence Length: 236

• Applicable Applications for IGF-2 elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration weretested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration weretested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF-2 elisa kit

Principle of the Assay: Rabbit Rabbit IGF-2 ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Rabbit IGF-2 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A25-E91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rabbit IGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rabbit Rabbit IGF-2 amount of sample captured in plate.

Background: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland. The rabbit IGF-II gene is located on chromosome 11. The standard product used in this kit is recombinant rabbit IGF-2, consisting of 67 amino acids in mature form with the molecular mass of 7.5KDa. IGF-1 and IGF-2 share high homology.

Product Categories/Family for IGF-2 elisa kit

Signal Transduction; Growth Factors/Hormones; insulin/insulin-like; Cancer; Growth Factors; insulin and insulin-like; Developmental Biology; post embryonic development; growth Hormones

NCBI and Uniprot Product Information

• NCBI GI #: 4504609
• NCBI GeneID: 3481
• NCBI Accession #: NP_000603.1
• NCBI GenBank Nucleotide #: NM_000612.5
• UniProt Accession #: P01344; P78449; Q14299; Q1WM26; Q9UC68; Q9UC69; B3KX48; B7WP08; C9JAF2; E3UN45
• Molecular Weight: 20140 MW
• NCBI Official Full Name: insulin-like growth factor II isoform 1 preproprotein
• NCBI Official Synonym Full Names: insulin like growth factor 2
• NCBI Official Symbol: IGF2
• NCBI Official Synonym Symbols: GRDF; IGF-II; PP9974; C11orf43
• NCBI Protein Information: insulin-like growth factor II
• UniProt Protein Name: Insulin-like growth factor II
• UniProt Gene Name: IGF2
• UniProt Synonym Gene Names: IGF-II

NCBI Description

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (). In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable).