CD4 recombinant antibody

Anti-CD4 [13B8.2]

Cat. Num. AAA488179

• Gene Names: CD4; CD4mut
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay
• Purity: Protein A Affinity Purified
• Synonyms: CD4; Anti-CD4 [13B8.2]; CD4 recombinant antibody

• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Clone Number: 13B8.2
• Specificity: Recognizes human CD4.
• Purity/Purification: Protein A Affinity Purified
• Sequence Length: 458

• Applicable Applications for CD4 recombinant antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin, ELISA (EIA), Flow Cytometry (FC/FACS)
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Immunogen: Human HSB2-T cells.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Flow Cytometry (FC/FACS)

( Flow-cytometry using anti-CD4 antibody 13B8.2 Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of 13B8.2 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)

Related Product Information for CD4 recombinant antibody

Recombinant monoclonal antibody to CD4. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 13B8.2. This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Product Categories/Family for CD4 recombinant antibody

Immunology

References

Bes et al. Efficient CD4 binding and immunosuppressive properties of the 13B8.2 monoclonal antibody are displayed by its CDR-H1-derived peptide CB1 FEBS Lett 2002 Feb 13; 512(1-3):354. PMID:11707270

NCBI and Uniprot Product Information

• NCBI GI #: 116013
• NCBI GeneID: 920
• UniProt Accession #: P01730; Q4ZGK2; Q5U066; Q9UDE5; B2R737; D3DUS5
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: early endosome; endoplasmic reticulum membrane; external side of plasma membrane; lipid raft; plasma membrane

Molecular Function: enzyme binding; glycoprotein binding; MHC class II protein binding; protein binding; protein homodimerization activity; protein kinase binding; receptor activity; transmembrane receptor activity; zinc ion binding

Biological Process: cell surface receptor linked signal transduction; entry into host cell; enzyme linked receptor protein signaling pathway; induction by virus of cell-cell fusion in host; maintenance of cellular protein localization; positive regulation of protein kinase activity; regulation of defense response to virus by virus; regulation of T cell activation; signal transduction; T cell costimulation; T cell differentiation; T cell receptor signaling pathway; T cell selection; viral envelope fusion with host membrane

Disease: Okt4 Epitope Deficiency