Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-CD4 research biosimilar antibody Clenoliximab (CE9.1, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD4 recombinant antibody
Anti-CD4 [CE9.1 (Clenoliximab)]
Gene Names
CD4; CD4mut
Reactivity
Human, Chimpanzee
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Protein A Affinity Purified
Synonyms
CD4; Anti-CD4 [CE9.1 (Clenoliximab)]; p55; T cell surface antign T4/Leu-3.; CD4 recombinant antibody
Reactivity
Human, Chimpanzee
Isotype
Rabbit IgG, lambda
Clone Number
CE9.1 (Clenoliximab)
Specificity
The antibody binds to human CD4 with a Kd of 1.0 nM and an ED50 of 0.2 ug/ml.
Purity/Purification
Protein A Affinity Purified
Sequence Length
71
Applicable Applications for CD4 recombinant antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Block, SPR, Immunohistochemistry (IHC)
Application Notes
The antibody binds specifically to CD4, a molecule which is part of the immunolgobulin super-family, expressed on the surface of helper T cells. The CD4 molecule acts as a co-receptor in the activation of T cells, aiding the interaction between the TCR and MHC II of the antigen-presenting cell. The antibody is classified as "primatized", as the variable regions of the light and heavy chains are obtained from primates, while the lamda light chain and 4 heavy chain constant domains are human. The antibody was engineered from the primatized IgG1 monoclonal antibody Keliximab by substituting two key residues. The role of the antibody as an immunosuppressor has been tested in vivo, by intravenous administration in chimpanzees and human volunteers. The antibody reached Phase II clinical trials with Biogen-Idec.
Buffer Composition
PBS only with 0.02% Proclin 300.
Immunogen
Domain 1 of human CD4.
Chimeric Use Statement
NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 (primatized) format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-CD4 research biosimilar antibody Clenoliximab (CE9.1, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-CD4 research biosimilar antibody Clenoliximab (CE9.1, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Related Product Information for CD4 recombinant antibody
Recombinant monoclonal antibody to CD4. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma CE9.1 (Clenoliximab). NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 (primatized) format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for CD4 recombinant antibody
References
Newman et al. Primatization of recombinant antibodies for immunotherapy of human diseases: a macaque/human chimeric antibody against human CD4. Biotechnology (NY). 1992 Nov; 10(11):1455-60. PMID:1369023
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
7,844 Da
NCBI Official Full Name
CD4, partial
NCBI Official Synonym Full Names
CD4 molecule
NCBI Official Symbol
CD4
NCBI Official Synonym Symbols
CD4mut
NCBI Protein Information
T-cell surface glycoprotein CD4
UniProt Protein Name
CD4 protein
Protein Family
UniProt Gene Name
CD4
UniProt Entry Name
Q13969_HUMAN
NCBI Description
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
Research Articles on CD4
Similar Products
Product Notes
The CD4 cd4 (Catalog #AAA488200) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD4 [CE9.1 (Clenoliximab)] reacts with Human, Chimpanzee and may cross-react with other species as described in the data sheet. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Block, SPR, Immunohistochemistry (IHC). The antibody binds specifically to CD4, a molecule which is part of the immunolgobulin super-family, expressed on the surface of helper T cells. The CD4 molecule acts as a co-receptor in the activation of T cells, aiding the interaction between the TCR and MHC II of the antigen-presenting cell. The antibody is classified as "primatized", as the variable regions of the light and heavy chains are obtained from primates, while the lamda light chain and 4 heavy chain constant domains are human. The antibody was engineered from the primatized IgG1 monoclonal antibody Keliximab by substituting two key residues. The role of the antibody as an immunosuppressor has been tested in vivo, by intravenous administration in chimpanzees and human volunteers. The antibody reached Phase II clinical trials with Biogen-Idec. Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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