Western Blot (WB)
(Anti-HEMGN Antibody (C-term) at 1:2000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human HEMGN Polyclonal Antibody | anti-HEMGN antibody
HEMGN Antibody (C-term)
Gene Names
HEMGN; NDR; EDAG; CT155; EDAG-1
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HEMGN; Polyclonal Antibody; HEMGN Antibody (C-term); Hemogen; Erythroid differentiation-associated gene protein; EDAG-1; Hemopoietic gene protein; Negative differentiation regulator protein; EDAG; NDR; anti-HEMGN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HEMGN antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 355-389 amino acids from the C-terminal region of human HEMGN.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
484
Applicable Applications for anti-HEMGN antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-HEMGN Antibody (C-term) at 1:2000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-HEMGN Antibody (C-term) at 1:2000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of lysate from Jurkat cell line, using HEMGN Antibody (C-term). MBS9209013 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Western Blot (WB)
(Western blot analysis of lysate from Jurkat cell line, using HEMGN Antibody (C-term). MBS9209013 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Related Product Information for anti-HEMGN antibody
Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).
Product Categories/Family for anti-HEMGN antibody
References
Yang L.V.,et al.Mech. Dev. 104:105-111(2001).
Yang L.V.,et al.Dev. Dyn. 228:606-616(2003).
Li C.Y.,et al.Cell Death Differ. 11:1299-1308(2004).
Liu C.C.,et al.J. Biomed. Sci. 11:104-116(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Yang L.V.,et al.Dev. Dyn. 228:606-616(2003).
Li C.Y.,et al.Cell Death Differ. 11:1299-1308(2004).
Liu C.C.,et al.J. Biomed. Sci. 11:104-116(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55341
NCBI Official Full Name
hemogen
NCBI Official Synonym Full Names
hemogen
NCBI Official Symbol
HEMGN
NCBI Official Synonym Symbols
NDR; EDAG; CT155; EDAG-1
NCBI Protein Information
hemogen
UniProt Protein Name
Hemogen
Protein Family
UniProt Gene Name
HEMGN
UniProt Synonym Gene Names
EDAG; NDR; EDAG-1
UniProt Entry Name
HEMGN_HUMAN
Uniprot Description
HEMGN: Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 9q22.33
Cellular Component: nucleoplasm
Molecular Function: protein binding
Biological Process: regulation of osteoblast differentiation; multicellular organismal development; cell differentiation
Research Articles on HEMGN
Similar Products
Product Notes
The HEMGN hemgn (Catalog #AAA9209013) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HEMGN Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HEMGN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the HEMGN hemgn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HEMGN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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