IL-2R recombinant antibody

Anti-IL-2R [Daclizumab]

Cat. Num. AAA488158

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A Affinity Purified
• Synonyms: IL-2R; Anti-IL-2R [Daclizumab]; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL-2-RA; IL2-RA; TAC antigen; p55; CD25; IL-2R recombinant antibody

• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Specificity: Binds to p55 chain of IL2R on human T cells, Tac antigen, which is expressed on T cells activated by mitogens, soluble antigens, and alloantigens but not on resting T cells, thymocytes, B cells, monocytes, activated B cells, leukemic T cell blasts from patients with acute lymphoblastic leukemia, and long-term T cell lines.
• Purity/Purification: Protein A Affinity Purified
• Sequence Length: 21

• Applicable Applications for IL-2R recombinant antibody: Flow Cytometry (FC/FACS), Block
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Immunogen: Cultured T-cells derived from a patient with Mycosis fungoides.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Flow Cytometry (FC/FACS)

( Flow-cytometry using the anti-CD25 (IL2R) research biosimilar antibody Daclizumab Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Daclizumab at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)

Flow Cytometry (FC/FACS)

( Flow-cytometry using anti-IL-2R antibody Daclizumab Cynomolgus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Daclizumab at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FlowJo single-cell flow cytometer.)

Flow Cytometry (FC/FACS)

( Flow-cytometry using anti-IL-2R antibody Daclizumab Rhesus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Daclizumab at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FlowJo single-cell flow cytometer.)

Related Product Information for IL-2R recombinant antibody

Recombinant monoclonal antibody to IL-2R. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the therapeutic antibody Daclizumab. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Product Categories/Family for IL-2R recombinant antibody

Biosimilar

References

Queen C, Schneider WP, Selick HE, Payne PW, Landolfi NF, Duncan JF, Avdalovic NM, Levitt M, Junghans R, Waldmann TA. A humanized antibody that binds to the interleukin 2 receptor. Proc Natl Acad Sci U S A. 1989 Dec; 86(24):10029-33. PMID:2513570

NCBI and Uniprot Product Information

• NCBI GI #: 554168
• NCBI Official Full Name: interleukin 2 receptor, partial