WAVE Phospho-Regulation Antibody Sampler Kit | WAVE antibody sampler kit
WAVE Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
WAVE Phospho-Regulation; WAVE antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for WAVE antibody sampler kit
Western Blot (WB), ELISA (EIA)
Preparation and Storage
Store all at -20 degree C. Stable for 1 year.
Related Product Information for WAVE antibody sampler kit
Kit Summary: The WAVE phospho-regulation antibody sampler kit can be used to examine phosphorylation of WAVE1 at Tyr-125 andTyr-150, as well as the conserved sites in WAVE2 and WAVE3. The kit also includes polyclonal antibodies to monitortotal expression levels of WAVE1 and WAVE2.
Background: The Wiskott-Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletalorganization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVEs 1, 2, and 3. WAVEproteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation toinduction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosinephosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required forWAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl mayenhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosinephosphorylation may be important for controlling specific activities of WAVE proteins.
Background: The Wiskott-Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletalorganization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVEs 1, 2, and 3. WAVEproteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation toinduction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosinephosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required forWAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl mayenhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosinephosphorylation may be important for controlling specific activities of WAVE proteins.
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Product Notes
The Human, Rat, Mouse WAVE (Catalog #AAA474651) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474651 Antibody Sampler Kit recognizes Human, Rat, Mouse WAVE. AAA Biotech's WAVE Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the WAVE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WAVE Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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