Rabbit anti-Human TBCE Polyclonal Antibody | anti-TBCE antibody

TBCE (Tubulin-specific Chaperone E, Tubulin-folding Cofactor E)

Cat. Num. AAA649564

• Gene Names: TBCE; HRD; KCS; KCS1; pac2
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Serum; Serum
• Synonyms: TBCE; Polyclonal Antibody; TBCE (Tubulin-specific Chaperone E; Tubulin-folding Cofactor E); Anti -TBCE (Tubulin-specific Chaperone E; anti-TBCE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TBCE.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid.
• Sequence: MSDTLTADVIGRRVEVNGEHATVRFAGVVPPVAGPWLGVEWDNPERGKHDGSHEGTVYFKCRHPTGGSFIRPNKVNFGTDFLTAIKNRYVLEDGPEEDRKEQIVTIGNKPVETIGFDSIMKQQSQLSKLQEVSLRNCAVSCAGEKGGVAEACPNIRKVDLSKNLLSSWDEVIHIADQLRHLEVLNVSENKLKFPSGSVLTGTLSVLKVLVLNQTGITWAEVLRCVAGCPGLEELYLESNNIFISERPTDVLQTVKLLDLSSNQLIDENQLYLIAHLPRLEQLILSDTGISSLHFPDAGIGCKTSMFPSLKYLVVNDNQISQWSFFNELEKLPSLRALSCLRNPLTKEDKEAETARLLIIASIGQLKTLNKCEILPEERRRAELDYRKAFGNEWKQAGGHKDPEKNRLSEEFLTAHPRYQFLCLKYGAPEDWELKTQQPLMLKNQLLTLKIKYPHQLDQKVLEKQLPGSMTIQKVKGLLSRLLKVPVSDLLLSYESPKKPGREIELENDLKSLQFYSVENGDCLLVRW

• Applicable Applications for anti-TBCE antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.
• Immunogen: Full length human TBCE, aa1-527 (NP_003184.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of TBCE expression in transfected 293T cell line by TBCE polyclonal antibody. Lane 1: TBCE transfected lysate (59.3kD). Lane 2: Non-transfected lysate.)

Immunoprecipitation (IP)

(Immunoprecipitation of TBCE transfected lysate using TBCE rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with TBCE purified mouse polyclonal antibody/)

Related Product Information for anti-TBCE antibody

Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state.

Product Categories/Family for anti-TBCE antibody

Antibodies; Abs to Tubulin

NCBI and Uniprot Product Information

• NCBI GI #: 118442828
• NCBI GeneID: 6905
• NCBI Accession #: NP_001072983.1
• NCBI GenBank Nucleotide #: NM_001079515.1
• UniProt Accession #: Q15813
• Molecular Weight: 59,346 Da
• NCBI Official Full Name: tubulin-specific chaperone E
• NCBI Official Synonym Full Names: tubulin folding cofactor E
• NCBI Official Symbol: TBCE
• NCBI Official Synonym Symbols: HRD; KCS; KCS1; pac2
• NCBI Protein Information: tubulin-specific chaperone E; Kenny-Caffey syndrome; tubulin-folding cofactor E
• UniProt Protein Name: Tubulin-specific chaperone E
• Protein Family: Tubulin-specific chaperone
• UniProt Gene Name: TBCE
• UniProt Entry Name: TBCE_HUMAN

NCBI Description

Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Tubulin-folding protein; involved in the second step of the tubulin folding pathway. Seems to be implicated in the maintenance of the neuronal microtubule network. Involved in regulation of tubulin heterodimer dissociation. Ref.5

Subunit structure: Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers.

Subcellular location: Cytoplasm

By similarity. Cytoplasm › cytoskeleton

By similarity.

Involvement in disease: Hypoparathyroidism-retardation-dysmorphism syndrome (HRD) [MIM:241410]: Autosomal recessive disorder reported almost exclusively in Middle Eastern populations.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10Kenny-Caffey syndrome 1 (KCS1) [MIM:244460]: An autosomal recessive form of Kenny-Caffey syndrome, a disorder characterized by impaired skeletal development with small and dense bones, short stature, and primary hypoparathyroidism with hypocalcemia. Clinical features include cortical thickening and medullary stenosis of the tubular bones, delayed closure of fontanels, defective dentition, small eyes with hypermetropia, and frontal bossing with a triangular face.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10

Sequence similarities: Belongs to the TBCE family.Contains 1 CAP-Gly domain.Contains 7 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.