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Smad3 recombinant protein
Smad3 Protein, Human, Mouse, Rat, Recombinant (His & GST Tag)
Gene Names
Smad3; Madh3; AU022421
Purity
>85% as determined by SDS-PAGE
Synonyms
Smad3; Smad3 Protein; Human; Mouse; Rat; Recombinant (His & GST Tag); Human/Mouse/Rat SMAD3 Protein (His & GST Tag); SMAD family member 3; Smad3 recombinant protein
Host
Baculovirus-Insect Cells
Purity/Purification
>85% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 500mM NaCl, 2mM GSH, 10% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Ser425
Species
Human, Mouse, Rat
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Met
Tag
N-GST & His
Protein Construction
A DNA sequence encoding the human/mouse/rat SMAD3 (NP_058049.3)(Met1-Ser425) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus. Human, Mouse and Rat sequences are identical.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for Smad3 recombinant protein
Background: SMAD3 belongs to the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD3 is involved in cell signalling. It modulates signals of activin and TGFbeta's. Binding of SMAD3 with SMAD4 enables its transmigration into the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD3 is a receptor-regulated SMAD (R-SMAD). In mice, mutation of SMAD3 has been linked to colorectal adenocarcinoma, increased systemic inflammation, and accelerated wound healing. Increased SMAD3 activity has been implicated in the pathogenesis of scleroderma. Smad3 is also a multifaceted regulator in adipose physiology and the pathogenesis of obesity and type 2 diabetes.
Product Categories/Family for Smad3 recombinant protein
References
Tan. et al., 2011, Diabetes. 60 (2): 464-76. Yang X. et al., 1999, Nat Cell Biol. 1 (5): 260-6. Zhu Y. et al., 1998, Cell. 94 (6): 703-14. Feng X. et al., 1996, Nature. 383 (6596): 168-72.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,081 Da
NCBI Official Full Name
mothers against decapentaplegic homolog 3
NCBI Official Synonym Full Names
SMAD family member 3
NCBI Official Symbol
Smad3
NCBI Official Synonym Symbols
Madh3; AU022421
NCBI Protein Information
mothers against decapentaplegic homolog 3
UniProt Protein Name
Mothers against decapentaplegic homolog 3
Protein Family
UniProt Gene Name
Smad3
UniProt Synonym Gene Names
Madh3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; mMad3; SMAD 3; Smad3
Uniprot Description
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator ().
Research Articles on Smad3
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Product Notes
The Smad3 smad3 (Catalog #AAA8123079) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ser42 5. It is sometimes possible for the material contained within the vial of "Smad3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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