Testing Data
Rabbit WAVE (Tyr-150) Polyclonal Antibody
WAVE (Tyr-150), phospho-specific [Conserved Site]
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot
Purity
Affinity Purified
Synonyms
WAVE (Tyr-150); Polyclonal Antibody; phospho-specific [Conserved Site]; Wiskott-Aldrich syndrome verproline-homologous protein 2; Scar; anti-WAVE (Tyr-150) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to phospho-WAVE (Tyr-125) and unphosphorylated WAVE (Tyr-150) peptides before affinity purification using phospho-WAVE (Tyr-150) peptide. In western blots, the antibody detects a 78 kDa* corresponding to phosphorylated WAVE2 in K562 cells treated with pervanadate and a 80 kDa band corresponding to WAVE1 in HUVEC treated with pervanadate. It also recognizes an unidentified band near 50 kDa in K562 cells.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-WAVE (Tyr-150) antibody
ELISA, Western Blot
Application Notes
ELISA: 1:2000
Western Blot: 1:1000
Western Blot: 1:1000
Immunogen
Phospho-WAVE (Tyr-150) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-150 in human WAVE2. This sequence has high homology with similar regions in rat and mouse WAVE2, and has homology to the conserved sites in WAVE1 (Tyr-151) and WAVE3 (Tyr-151).
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for anti-WAVE (Tyr-150) antibody
The Wiskott-Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVEs 1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an Nterminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.
References
• Ardern, H. et al. (2006) Cell Motil. Cytosk. 63:6.
• Leng, Y. et al. (2005) PNAS 102(4):1098.
• Suetsugu, S. et al. (1999) Bioch. Biophs. Res. Comm. 260:296.
• Miki, H. et al. (1999) J Biol. Chem. 274(39):27605.
• Leng, Y. et al. (2005) PNAS 102(4):1098.
• Suetsugu, S. et al. (1999) Bioch. Biophs. Res. Comm. 260:296.
• Miki, H. et al. (1999) J Biol. Chem. 274(39):27605.
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Product Notes
The WAVE (Tyr-150) (Catalog #AAA472068) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WAVE (Tyr-150), phospho-specific [Conserved Site] reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's WAVE (Tyr-150) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. ELISA: 1:2000 Western Blot: 1:1000. Researchers should empirically determine the suitability of the WAVE (Tyr-150) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WAVE (Tyr-150), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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