Rabbit WAVE1 (N-terminal region) Polyclonal Antibody | anti-WASF1 antibody

WAVE1 (N-terminal region)

Cat. Num. AAA472069

• Gene Names: WASF1; WAVE; SCAR1; WAVE1; FLJ31482; KIAA0269
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified
• Synonyms: WAVE1 (N-terminal region); Polyclonal Antibody; Wiskott-Aldrich syndrome verproline-homologous protein 1; Scar1; WASF1; anti-WASF1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using WAVE1 (Nterminal region) peptide (without carrier). The antibody detects an 80 kDa* protein corresponding to the molecular mass of WAVE1 on SDS-PAGE immunoblots of human SYF cSrc-transformed, HUVEC, and K562 cells, as well as rat PC12 cells and mouse C2C12 cells.
• Purity/Purification: Affinity Purified
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-WASF1 antibody: ELISA, Western Blot
• Application Notes: ELISA: 1:2000; WB: 1:1000; ICC: 1:200
• Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal half of human WAVE1. This sequence has high homology with similar regions in rat and mouse WAVE1, and has less than 50% homology to similar regions in human WAVE2 and WAVE3.
• Preparation and Storage: Store at -20°C. Stable for 1 year.

Testing Data

Immunocytochemical (ICC)

(Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)

Related Product Information for anti-WASF1 antibody

The Wiskott-Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVE1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an Nterminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.

References

• Ardern, H. et al. (2006) Cell Motil. Cytosk. 63:6.
• Leng, Y. et al. (2005) PNAS 102(4):1098.
• Suetsugu, S. et al. (1999) Bioch. Biophs. Res. Comm. 260:296.
• Miki, H. et al. (1999) J Biol. Chem. 274(39):27605.

NCBI and Uniprot Product Information

• NCBI GI #: 68161504
• NCBI GeneID: 8936
• NCBI Accession #: NP_001020107.1
• NCBI GenBank Nucleotide #: NM_001024936.1
• UniProt Accession #: Q92558; Q5SZK7; E1P5F2; Q5SZK3; Q5SZK4; Q5SZK5
• NCBI Official Full Name: wiskott-Aldrich syndrome protein family member 1
• NCBI Official Synonym Full Names: WAS protein family, member 1
• NCBI Official Symbol: WASF1
• NCBI Official Synonym Symbols: WAVE; SCAR1; WAVE1; FLJ31482; KIAA0269
• NCBI Protein Information: wiskott-Aldrich syndrome protein family member 1; protein WAVE-1; OTTHUMP00000016990; OTTHUMP00000016992; WASP family protein member 1; homology of dictyostelium scar 1; verprolin homology domain-containing protein 1
• UniProt Protein Name: Wiskott-Aldrich syndrome protein family member 1
• Protein Family: Wiskott-Aldrich syndrome protein family
• UniProt Gene Name: WASF1
• UniProt Synonym Gene Names: KIAA0269; SCAR1; WAVE1
• UniProt Entry Name: WASF1_HUMAN

NCBI Description

The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Uniprot Description

Function: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.

Subunit structure: Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1

By similarity. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Ref.7 Ref.8

Subcellular location: Cytoplasm › cytoskeleton. Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.

Tissue specificity: Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

Domain: Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Sequence similarities: Belongs to the SCAR/WAVE family.Contains 1 WH2 domain.

Sequence caution: The sequence BAA13399.2 differs from that shown. Reason: Erroneous initiation.