Typical Testing Data/Standard Curve (for reference only)
Human Vitamin D3 ELISA Kit
Vitamin D3 (human) ELISA kit
Reactivity
Human
Synonyms
Vitamin D3; Vitamin D3 (human) ELISA kit; Human Vitamin D3 (Cholecalciferol) ELISA kit; Vitamin D3 elisa kit
Reactivity
Human
Application Notes
Quantitative protein detection, establishing normal range, validation of antibody array results
Samples
Serum & plasma (EDTA/Citrate), Urine, Cell culture medium, Tissue homogenates and Cell lysates
Assay Type
Sandwich
Detection Method
Absorbance (450 nm)
Features & Benefits
Easy, convenient and time-saving method to assay for human vitamin D3.
Detection Range:200 - 800 ug/L.
This ELISA kit shows no species cross-reactivity.
Detection Range:200 - 800 ug/L.
This ELISA kit shows no species cross-reactivity.
Preparation and Storage
4 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Vitamin D3 elisa kit
Product Information: Vitamin D3 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human Vitamin D3 (cholecalciferol) in serum, plasma, urine, cell culture media and cell/tissue lysates. Detection Range:200 - 800 ug/L. 100 assays.
Background: Vitamin D3 (Cholecalciferol) is found in human epidermis and dermis. MyBioSource's human Vit. D3 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human Vit. D3 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Vit. D3 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Vit. D3 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 20 - 800 ug/L.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-human
Application- Quantitative protein detection, establishing normal range.
Background: Vitamin D3 (Cholecalciferol) is found in human epidermis and dermis. MyBioSource's human Vit. D3 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human Vit. D3 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Vit. D3 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Vit. D3 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 20 - 800 ug/L.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-human
Application- Quantitative protein detection, establishing normal range.
Product Categories/Family for Vitamin D3 elisa kit
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Product Notes
The Human Vitamin D3 (Catalog #AAA843322) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA843322 ELISA Kit recognizes Human Vitamin D3. Quantitative protein detection, establishing normal range, validation of antibody array results. Researchers should empirically determine the suitability of the Vitamin D3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vitamin D3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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