Typical Testing Data/Standard Curve (for reference only)
Human Apolipoprotein E4/Pan-ApoE ELISA Kit
Apolipoprotein E4 / Pan-ApoE (human) ELISA Kit
Reactivity
Human
Synonyms
Apolipoprotein E4/Pan-ApoE; Apolipoprotein E4 / Pan-ApoE (human) ELISA Kit; Apolipoprotein E4/Pan-ApoE elisa kit
Reactivity
Human
Application Notes
Quantitative measurement of human Apolipoprotein E4 (ApoE4)
Samples
Serum and plasma, Cell lysate and Cell Culture Supernatant, Cerebrospinal Fluid (CSF)
Detection Method
Absorbance (450 nm)
Features & Benefits
Easy, convenient and time-saving method to assay for human ApoE4.
Sensitivity: 25 ng/ml. •Detection range: 50 ng to 800 ng/ml.
Sensitivity: 25 ng/ml. •Detection range: 50 ng to 800 ng/ml.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Apolipoprotein E4/Pan-ApoE elisa kit
Product Information: Apolipoprotein E4/Pan-ApoE (human) ELISA Kit: Rapid & convenient assay for quantitative measurement of human ApoE4 in serum, plasma, cell lysate & cell culture supernatant, & cerebrospinal fluid. Detection range: 50 ng to 800 ng/ml. 100 assays.
Background: MyBioSource's Human Apolipoprotein E4 (ApoE4) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for quantitative measurement of human ApoE4. ApoE transports lipoproteins, fat-soluble vitamins, and cholesterol via the lymph system to the blood. ApoE exists as three major isoforms, including ApoE2, ApoE3, and ApoE4. Recently, ApoE4 has been implicated in Alzheimer's disease (AD). ApoE4 is the first risk gene identified in Alzheimer's research, and remains the gene with strongest impact. Everyone inherits a copy of ApoE gene from each parent. Those who inherit one copy of ApoE4 have an increased risk of developing AD (about a quarter of the human population). Those who inherit two copies of ApoE4 have an even higher risk (about 2% of humans with up to 10 times higher risk). In addition to raising risk, ApoE4 may tend to make AD symptoms appear at a younger age. The assay employs an antibody specific for human ApoE4 coated on a 96-well plate. Standard and samples are pipetted into wells and ApoE4 present in the sample is bound to the wells by immobilized antibody. Wells are washed and human ApoE4 specific detection antibody is added. After washing away unbound detection antibody, HRP-conjugate is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of bound ApoE4. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 25 ng/ml and detection range is from 50 ng to 800 ng/ml. Recovery inside this range is between 83 and 104% (average recovery is 93%). The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- human
Application- Quantitative measurement of human Apolipoprotein E4 (ApoE4)
Background: MyBioSource's Human Apolipoprotein E4 (ApoE4) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for quantitative measurement of human ApoE4. ApoE transports lipoproteins, fat-soluble vitamins, and cholesterol via the lymph system to the blood. ApoE exists as three major isoforms, including ApoE2, ApoE3, and ApoE4. Recently, ApoE4 has been implicated in Alzheimer's disease (AD). ApoE4 is the first risk gene identified in Alzheimer's research, and remains the gene with strongest impact. Everyone inherits a copy of ApoE gene from each parent. Those who inherit one copy of ApoE4 have an increased risk of developing AD (about a quarter of the human population). Those who inherit two copies of ApoE4 have an even higher risk (about 2% of humans with up to 10 times higher risk). In addition to raising risk, ApoE4 may tend to make AD symptoms appear at a younger age. The assay employs an antibody specific for human ApoE4 coated on a 96-well plate. Standard and samples are pipetted into wells and ApoE4 present in the sample is bound to the wells by immobilized antibody. Wells are washed and human ApoE4 specific detection antibody is added. After washing away unbound detection antibody, HRP-conjugate is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of bound ApoE4. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 25 ng/ml and detection range is from 50 ng to 800 ng/ml. Recovery inside this range is between 83 and 104% (average recovery is 93%). The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- human
Application- Quantitative measurement of human Apolipoprotein E4 (ApoE4)
Product Categories/Family for Apolipoprotein E4/Pan-ApoE elisa kit
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Product Notes
The Human Apolipoprotein E4/Pan-ApoE (Catalog #AAA843430) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA843430 ELISA Kit recognizes Human Apolipoprotein E4/Pan-ApoE. Quantitative measurement of human Apolipoprotein E4 (ApoE4). Researchers should empirically determine the suitability of the Apolipoprotein E4/Pan-ApoE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apolipoprotein E4/Pan-ApoE, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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