Human Riboflavin Kinase (RFK) RTU ELISA Kit | RFK rtu elisa kit

Human Riboflavin Kinase (RFK) ELISA Kit

Cat. Num. AAA4502228

• Gene Names: RFK; RIFK
• Reactivity: Human
• Synonyms: Riboflavin Kinase (RFK); Human Riboflavin Kinase (RFK) ELISA Kit; RIFK; Flavokinase; ATP:riboflavin 5'-phosphotransferase; RFK rtu elisa kit

• Reactivity: Human
• Sequence Length: 155

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.27ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RFK rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RFK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RFK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RFK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RFK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 269973877
• NCBI GeneID: 55312
• NCBI Accession #: NP_060809.3
• NCBI GenBank Nucleotide #: NM_018339.5
• UniProt Accession #: Q969G6; Q5JSG9; Q9NUT7
• Molecular Weight: 17,623 Da
• NCBI Official Full Name: riboflavin kinase
• NCBI Official Synonym Full Names: riboflavin kinase
• NCBI Official Symbol: RFK
• NCBI Official Synonym Symbols: RIFK
• NCBI Protein Information: riboflavin kinase
• UniProt Protein Name: Riboflavin kinase
• UniProt Gene Name: RFK

NCBI Description

Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009]

Uniprot Description

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.