Typical Testing Data/Standard Curve (for reference only)
Human TGF-alpha ELISA Kit | TGF-alpha elisa kit
TGF-alpha (human) ELISA Kit
Reactivity
Human
Synonyms
TGF-alpha; TGF-alpha (human) ELISA Kit; Transforming growth factor alpha (human) ELISA Kit; TGF-alpha elisa kit
Reactivity
Human
Sequence Length
173
Application Notes
Quantitative protein detection, establishing normal range.
Samples
Serum & plasma (heparin, EDTA), Cell Culture Supernatants, Human milk
Assay Type
Sandwich
Detection Method
Absorbance (450 nm)
Features & Benefits
Easy, convenient and time-saving method to assay for human TGF-alpha.
Detection Range: 15.6-1000 pg/ml. Sensitivity: < 1 pg/ml.
This ELISA kit shows cross-reactivity with TGFbeta2, TGFbeta3 & TGFbeta5 <1%.
Detection Range: 15.6-1000 pg/ml. Sensitivity: < 1 pg/ml.
This ELISA kit shows cross-reactivity with TGFbeta2, TGFbeta3 & TGFbeta5 <1%.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TGF-alpha elisa kit
Product Information: TGF-alpha (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human TGF-alpha in serum, plasma, cell culture supernatants & human milk. Detection Range: 15.6 pg/ml - 1000 pg/ml. 100 assays.
Background: MyBioSource's human TGF-alpha ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody from mouse specific for TGF-alpha coated on a 96-well plate. Standards and test samples are added to the wells and TGF-alpha present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for TGF-alpha is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human TGF-alpha captured onto the plate. This ELISA kit shows cross-reactivity with TGFbeta2, TGFbeta3 & TGFbeta5 <1%. Detection Range: 15.6 pg/ml - 1000 pg/ml. Sensitivity: < 1 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-human
Application- Quantitative protein detection, establishing normal range etc.
Background: MyBioSource's human TGF-alpha ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody from mouse specific for TGF-alpha coated on a 96-well plate. Standards and test samples are added to the wells and TGF-alpha present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for TGF-alpha is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human TGF-alpha captured onto the plate. This ELISA kit shows cross-reactivity with TGFbeta2, TGFbeta3 & TGFbeta5 <1%. Detection Range: 15.6 pg/ml - 1000 pg/ml. Sensitivity: < 1 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-human
Application- Quantitative protein detection, establishing normal range etc.
Product Categories/Family for TGF-alpha elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
19,279 Da
NCBI Official Full Name
TGF-alpha, partial
UniProt Protein Name
TGF-alpha
UniProt Gene Name
tgfa
UniProt Entry Name
D7RNZ2_GRYBI
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Product Notes
The Human TGF-alpha tgfa (Catalog #AAA843327) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA843327 ELISA Kit recognizes Human TGF-alpha. Quantitative protein detection, establishing normal range. Researchers should empirically determine the suitability of the TGF-alpha tgfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGF-alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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