Typical Testing Data/Standard Curve (for reference only)
Human uPAR ELISA Kit | uPAR elisa kit
uPAR ELISA
Reactivity
Human
Synonyms
uPAR; uPAR ELISA; uPAR elisa kit
Reactivity
Human
Samples
Human serum, plasma, body fluids, tissue lysates or cell culture supernates
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
<4pg/ml
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for uPAR elisa kit
Intended Uses: For detection of uPAR in human serum, plasma, body fluids, tissue lysates or cell culture supernates. For research use only
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-uPAR polyclonal antibody is pre-coated onto 96-well plates. And the biotin conjugated anti-uPAR polyclonal antibody is used as detection antibodies. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the uPAR amount of sample captured on the plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of uPAR can be calculated.!!Background/Introduction: The Urokinase receptor, also known as uPA receptor or uPAR or CD87 (Cluster of Differentiation 87), is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. uPAR was originally identified as a saturable binding site for urokinase on the cell surface. It consists of three different domains of the Ly-6/uPAR/ alpha-neurotoxin family. All three domains are necessary for high affinity binding of the primary ligand, urokinase. uPAR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. It is a key molecule in the regulation of cell-surface plasminogen activation and plays an important role in many normal as well as pathologic processes.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-uPAR polyclonal antibody is pre-coated onto 96-well plates. And the biotin conjugated anti-uPAR polyclonal antibody is used as detection antibodies. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the uPAR amount of sample captured on the plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of uPAR can be calculated.!!Background/Introduction: The Urokinase receptor, also known as uPA receptor or uPAR or CD87 (Cluster of Differentiation 87), is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. uPAR was originally identified as a saturable binding site for urokinase on the cell surface. It consists of three different domains of the Ly-6/uPAR/ alpha-neurotoxin family. All three domains are necessary for high affinity binding of the primary ligand, urokinase. uPAR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. It is a key molecule in the regulation of cell-surface plasminogen activation and plays an important role in many normal as well as pathologic processes.
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Product Notes
The Human uPAR (Catalog #AAA494830) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494830 ELISA Kit recognizes Human uPAR. It is sometimes possible for the material contained within the vial of "uPAR, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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