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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TALDO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

Rabbit anti-Human, Mouse TALDO1 Polyclonal Antibody | anti-TALDO1 antibody

TALDO1 Polyclonal Antibody

Gene Names
TALDO1; TAL; TALH; TAL-H; TALDOR
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
TALDO1; Polyclonal Antibody; TALDO1 Polyclonal Antibody; TAL; TALH; TAL-H; TALDOR; anti-TALDO1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
337
Applicable Applications for anti-TALDO1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human TALDO1
Calculated Molecular Weight
37kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TALDO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TALDO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using TALDO1 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using TALDO1 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-TALDO1 antibody
Transaldolase 1 is a key enzyme of the nonoxidative pentose phosphate pathway providing ribose-5-phosphate for nucleic acid synthesis and NADPH for lipid biosynthesis. This pathway can also maintain glutathione at a reduced state and thus protect sulfhydryl groups and cellular integrity from oxygen radicals. The functional gene of transaldolase 1 is located on chromosome 11 and a pseudogene is identified on chromosome 1 but there are conflicting map locations. The second and third exon of this gene were developed by insertion of a retrotransposable element. This gene is thought to be involved in multiple sclerosis.
Product Categories/Family for anti-TALDO1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
337
NCBI Official Full Name
Transaldolase
NCBI Official Synonym Full Names
transaldolase 1
NCBI Official Symbol
TALDO1
NCBI Official Synonym Symbols
TAL; TALH; TAL-H; TALDOR
NCBI Protein Information
transaldolase; glycerone transferase; dihydroxyacetone transferase
UniProt Protein Name
Transaldolase
Protein Family
UniProt Gene Name
TALDO1
UniProt Synonym Gene Names
TAL; TALDO; TALDOR
UniProt Entry Name
TALDO_HUMAN

NCBI Description

Transaldolase 1 is a key enzyme of the nonoxidative pentose phosphate pathway providing ribose-5-phosphate for nucleic acid synthesis and NADPH for lipid biosynthesis. This pathway can also maintain glutathione at a reduced state and thus protect sulfhydryl groups and cellular integrity from oxygen radicals. The functional gene of transaldolase 1 is located on chromosome 11 and a pseudogene is identified on chromosome 1 but there are conflicting map locations. The second and third exon of this gene were developed by insertion of a retrotransposable element. This gene is thought to be involved in multiple sclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

TALDO1: Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway. Defects in TALDO1 are the cause of transaldolase 1 deficiency (TALDO1 deficiency). It results in telangiectases of the skin, hepatosplenomegaly, and enlarged clitoris. Belongs to the transaldolase family. Type 1 subfamily.

Protein type: Transferase; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.2.1.2

Chromosomal Location of Human Ortholog: 11p15.5-p15.4

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: transaldolase activity

Biological Process: pentose-phosphate shunt; carbohydrate metabolic process; energy reserve metabolic process; xylulose biosynthetic process; pathogenesis

Disease: Transaldolase Deficiency

Research Articles on TALDO1

Similar Products

Product Notes

The TALDO1 taldo1 (Catalog #AAA9127746) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TALDO1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TALDO1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the TALDO1 taldo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TALDO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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