Typical Testing Data/Standard Curve (for reference only)
Human ErbB-2 ELISA Kit | ErbB-2 elisa kit
ErbB-2 ELISA
Gene Names
ERBB2; HER-2
Reactivity
Human
Synonyms
ErbB-2; ErbB-2 ELISA; ErbB-2 elisa kit
Reactivity
Human
Sequence Length
1259
Samples
Tissue Lysates Or Cell Culture Supernates
Assay Type
Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<2pg/ml
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ErbB-2 elisa kit
Intended Uses: For detection of Emmprin in tissue lysates or cell culture supernates. The significance of detection in human serum, plasma, body fluids is undetermined. For research use only.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Emmprin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Emmprin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Emmprin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Emmprin can be calculated!!Background/Introduction: Cluster of Differentiation 147 (CD147) also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or Basigin (BSG), is a member of the immunoglobulin superfamily, It interacts with fibroblasts and stimulates expression of MMPs, and plays an important role in tumor invasiveness and metastasis. This protein is a determinant for the Ok blood group system, and it has been shown to be an essential receptor on red blood cells for the human malaria parasite, Plasmodium falciparum.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Emmprin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Emmprin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Emmprin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Emmprin can be calculated!!Background/Introduction: Cluster of Differentiation 147 (CD147) also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or Basigin (BSG), is a member of the immunoglobulin superfamily, It interacts with fibroblasts and stimulates expression of MMPs, and plays an important role in tumor invasiveness and metastasis. This protein is a determinant for the Ok blood group system, and it has been shown to be an essential receptor on red blood cells for the human malaria parasite, Plasmodium falciparum.
Product Categories/Family for ErbB-2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
137,991 Da
NCBI Official Full Name
erbB-2
NCBI Official Symbol
ERBB2
NCBI Official Synonym Symbols
HER-2
NCBI Protein Information
receptor tyrosine-protein kinase erbB-2; c-erbB-2; p185erbB2; proto-oncogene c-ErbB-2
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-2
UniProt Gene Name
ERBB2
UniProt Entry Name
ERBB2_CANFA
Uniprot Description
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization ().
Research Articles on ErbB-2
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Product Notes
The Human ErbB-2 erbb2 (Catalog #AAA494769) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494769 ELISA Kit recognizes Human ErbB-2. It is sometimes possible for the material contained within the vial of "ErbB-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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