Human Cholesterol-25-Hydroxylase (CH25H) ELISA Kit | CH25H elisa kit

Human Cholesterol-25-Hydroxylase (CH25H) ELISA Kit

Cat. Num. AAA453808

• Gene Names: CH25H; C25H
• Reactivity: Human
• Synonyms: Cholesterol-25-Hydroxylase (CH25H); Human Cholesterol-25-Hydroxylase (CH25H) ELISA Kit; GPR129; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4; O3FAR1; Omega-3 fatty acid; CH25H elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CH25H. No significant cross-reactivity or interference between CH25H and analogues was observed.
• Sequence Length: 272

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.252ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CH25H were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CH25H were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CH25H elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CH25H in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CH25H. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CH25H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CH25H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CH25H in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502499
• NCBI GeneID: 9023
• NCBI Accession #: NP_003947.1
• NCBI GenBank Nucleotide #: NM_003956.3
• UniProt Accession #: O95992; B2RBY3
• Molecular Weight: 31,745 Da
• NCBI Official Full Name: cholesterol 25-hydroxylase
• NCBI Official Synonym Full Names: cholesterol 25-hydroxylase
• NCBI Official Symbol: CH25H
• NCBI Official Synonym Symbols: C25H
• NCBI Protein Information: cholesterol 25-hydroxylase
• UniProt Protein Name: Cholesterol 25-hydroxylase
• Protein Family: Cholesterol 25-hydroxylase
• UniProt Gene Name: CH25H
• UniProt Synonym Gene Names: h25OH

NCBI Description

This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.