Typical Testing Data/Standard Curve (for reference only)
Rat Toll Like Receptor 7 ELISA Kit | TLR7 elisa kit
Rat Toll Like Receptor 7 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TLR-7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLR-7 antibody and an TLR-7-HRP conjugate. The assay sample and buffer are incubated together with TLR-7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLR-7 concentration since TLR-7 from samples and TLR-7-HRP conjugate compete for the anti-TLR-7 antibody binding site. Since the number of sites is limited, as more sites are occupied by TLR-7 from the sample, fewer sites are left to bind TLR-7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR-7 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Jul 2008]
Uniprot Description
TLR7: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1. Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. Belongs to the Toll-like receptor family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: Xp22.3
Cellular Component: Golgi membrane; endoplasmic reticulum membrane; lysosome; endoplasmic reticulum; cytoplasm; plasma membrane; integral to membrane; endosome membrane; receptor complex; endosome
Molecular Function: siRNA binding; single-stranded RNA binding; transmembrane receptor activity; double-stranded RNA binding; drug binding
Biological Process: microglial cell activation; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; positive regulation of interferon-alpha biosynthetic process; positive regulation of NF-kappaB import into nucleus; toll-like receptor 7 signaling pathway; positive regulation of chemokine production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-8 production; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; I-kappaB phosphorylation; defense response to virus; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process
Research Articles on TLR7
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Product Notes
The Rat TLR7 tlr7 (Catalog #AAA753729) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA753729 ELISA Kit recognizes Rat TLR7. It is sometimes possible for the material contained within the vial of "Toll Like Receptor 7, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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