Typical Testing Data/Standard Curve (for reference only)
Human TNFbeta ELISA Kit | TNFbeta elisa kit
TNFbeta Human ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
TNFbeta; TNFbeta Human ELISA Kit; Human TNFbeta; TNFbeta elisa kit
Reactivity
Human
Applicable Applications for TNFbeta elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of TNFbeta in human serum, plasma, body fluids, tissue lysates or cell culture supernates.
Assay Type
Sandwich
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
< 5pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TNFbeta elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PCadherin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TNF beta polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TNFbeta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TNFbeta can be calculated.
Background: Tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin-alpha(LTA), is a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. It is a glycoprotein with a relative molecular mass (Mr) of 60,000-70,000. LTA is highly inducible, secreted, and exists as homotrimeric molecule. It forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. It is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
Background: Tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin-alpha(LTA), is a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. It is a glycoprotein with a relative molecular mass (Mr) of 60,000-70,000. LTA is highly inducible, secreted, and exists as homotrimeric molecule. It forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. It is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
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Product Notes
The Human TNFbeta (Catalog #AAA355425) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355425 ELISA Kit recognizes Human TNFbeta. AAA Biotech's TNFbeta can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of TNFbeta in human serum, plasma, body fluids, tissue lysates or cell culture supernates. Researchers should empirically determine the suitability of the TNFbeta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFbeta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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