Typical Testing Data/Standard Curve (for reference only)
Human uPA ELISA Kit | uPA elisa kit
uPA Human ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
uPA; uPA Human ELISA Kit; Human uPA; uPA elisa kit
Reactivity
Human
Applicable Applications for uPA elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of uPA in human serum, plasma, body fluids, tissue lysates or cell culture supernates.
Assay Type
Sandwich
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
< 5pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for uPA elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-uPA polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-uPA polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the uPA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of uPA can be calculated.
Background: Urokinase (trade name Abbokinase), also called urokinase-type plasminogen activator (uPA), is a 411-residue protein, consisting of three domains: the serine protease domain, the kringle domain, and the growth factor domain. It has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively.[1] Activation of plasmin triggers a proteolysis cascade that, depending on the physiological environment, participates in thrombolysis or extracellular matrix degradation. This links urokinase to vascular diseases and cancer.
Background: Urokinase (trade name Abbokinase), also called urokinase-type plasminogen activator (uPA), is a 411-residue protein, consisting of three domains: the serine protease domain, the kringle domain, and the growth factor domain. It has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively.[1] Activation of plasmin triggers a proteolysis cascade that, depending on the physiological environment, participates in thrombolysis or extracellular matrix degradation. This links urokinase to vascular diseases and cancer.
Similar Products
Product Notes
The Human uPA (Catalog #AAA355427) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355427 ELISA Kit recognizes Human uPA. AAA Biotech's uPA can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of uPA in human serum, plasma, body fluids, tissue lysates or cell culture supernates. Researchers should empirically determine the suitability of the uPA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "uPA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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