Human BMP-5 ELISA Kit | BMP-5 elisa kit

BMP-5 Human ELISA Kit

Cat. Num. AAA355388

• Reactivity: Human
• Applications: ELISA
• Synonyms: BMP-5; BMP-5 Human ELISA Kit; Human BMP-5; BMP-5 elisa kit

• Reactivity: Human

• Applicable Applications for BMP-5 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of BMP-5 in human serum, plasma, body fluids, tissue lysates or cell culture supernates.
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP-5 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- BMP-5 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- BMP-5 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BMP-5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BMP-5 can be calculated.

Background: Bone morphogenetic protein 5 (BMP-5) is a member of the transforming growth factor-beta superfamily of regulatory molecules. Bone morphogenetic proteins were originally identified by the ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site, and known for their ability to induce bone and cartilage development. BMP-5 is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. It may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. This protein also act as an important signaling molecule within the trabecular meshwork and optic nerve head, and may play a potential role in glaucoma pathogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 10835091
• NCBI GeneID: 653
• NCBI Accession #: NP_066551.1
• NCBI GenBank Nucleotide #: NM_021073.2
• UniProt Accession #: P22003; Q9H547; Q9NTM5
• Molecular Weight: 51,737 Da
• NCBI Official Full Name: bone morphogenetic protein 5 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 5
• NCBI Official Symbol: BMP5
• NCBI Protein Information: bone morphogenetic protein 5; BMP-5; bone morphogenenic protein-5
• UniProt Protein Name: Bone morphogenetic protein 5
• UniProt Gene Name: BMP5
• UniProt Synonym Gene Names: BMP-5
• UniProt Entry Name: BMP5_HUMAN

NCBI Description

This gene encodes a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. These proteins are synthesized as prepropeptides, cleaved, and then processed into dimeric proteins. This protein may act as an important signaling molecule within the trabecular meshwork and optic nerve head, and may play a potential role in glaucoma pathogenesis. This gene is differentially regulated during the formation of various tumors. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Induces cartilage and bone formation.

Subunit structure: Homodimer; disulfide-linked

By similarity.

Subcellular location: Secreted.

Tissue specificity: Expressed in the lung and liver.

Sequence similarities: Belongs to the TGF-beta family.