Typical Testing Data/Standard Curve (for reference only)
Mouse OPG ELISA Kit | OPG elisa kit
OPG Mouse ELISA Kit
Reactivity
Mouse
Applications
ELISA
Synonyms
OPG; OPG Mouse ELISA Kit; Mouse OPG; OPG elisa kit
Reactivity
Mouse
Applicable Applications for OPG elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of OPG in mouse serum, body fluids, tissue lysates or cell culture supernates.
Assay Type
Sandwich
Detection Range
93.8 pg/ml-6,000 pg/ml
Sensitivity
< 5pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for OPG elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-OPG polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-OPG polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the OPG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of OPG can be calculated.
Background: Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF), or tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), is a protein that in humans is encoded by the TNFRSF11B gene. It is a member of the tumor necrosis factor (TNF) receptor superfamily, and comprising 401 amino acid residues arranged into 7 structural domains. It is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL). OPG can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors. Elevated OPG levels has been reported in heart diseases, in Placebo effect serum responses in IBS patients and in severe mental disorders.
Background: Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF), or tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), is a protein that in humans is encoded by the TNFRSF11B gene. It is a member of the tumor necrosis factor (TNF) receptor superfamily, and comprising 401 amino acid residues arranged into 7 structural domains. It is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL). OPG can reduce the production of osteoclasts by inhibiting the differentiation of osteoclast precursors. Elevated OPG levels has been reported in heart diseases, in Placebo effect serum responses in IBS patients and in severe mental disorders.
Similar Products
Product Notes
The Mouse OPG (Catalog #AAA355246) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355246 ELISA Kit recognizes Mouse OPG. AAA Biotech's OPG can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of OPG in mouse serum, body fluids, tissue lysates or cell culture supernates. Researchers should empirically determine the suitability of the OPG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OPG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
