Mouse Toll-like receptor 6 ELISA Kit | TLR6 elisa kit

Mouse Toll-like receptor 6 ELISA Kit

Cat. Num. AAA763006

• Gene Names: TLR6; CD286
• Reactivity: Mouse
• Synonyms: Toll-like receptor 6; Mouse Toll-like receptor 6 ELISA Kit; TLR6/CD286/CD286 antigen/toll-like receptor 6; TLR6 elisa kit

• Reactivity: Mouse
• Sequence Length: 796

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TLR6 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse TLR6 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse TLR6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TLR6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMouse TLR6 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20143971
• NCBI GeneID: 10333
• NCBI Accession #: NP_006059.2
• NCBI GenBank Nucleotide #: NM_006068.4
• Molecular Weight: 55,828 Da
• NCBI Official Full Name: toll-like receptor 6
• NCBI Official Synonym Full Names: toll like receptor 6
• NCBI Official Symbol: TLR6
• NCBI Official Synonym Symbols: CD286
• NCBI Protein Information: toll-like receptor 6
• UniProt Protein Name: Toll-like receptor 6
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR6
• UniProt Entry Name: TLR6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor functionally interacts with toll-like receptor 2 to mediate cellular response to bacterial lipoproteins. A Ser249Pro polymorphism in the extracellular domain of the encoded protein may be associated with an increased of asthma is some populations.[provided by RefSeq, Jan 2011]

Uniprot Description

TLR6: Participates in the innate immune response to Gram- positive bacteria and fungi. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: Golgi apparatus; integral to plasma membrane; lipid raft; phagocytic vesicle membrane; plasma membrane

Molecular Function: diacylated lipoprotein binding; protein binding; protein heterodimerization activity; receptor activity; transmembrane receptor activity

Biological Process: activation of NF-kappaB-inducing kinase; defense response to bacterium; detection of diacylated bacterial lipoprotein; immune response; inflammatory response; innate immune response; interleukin-1 beta secretion; MyD88-dependent toll-like receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK activity; regulation of cytokine secretion; signal transduction; T-helper 1 type immune response; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 6 signaling pathway; toll-like receptor signaling pathway