Rabbit anti-Human, Mouse NXPH1 Polyclonal Antibody | anti-NXPH1 antibody

NXPH1, NT (NXPH1, NPH1, Neurexophilin-1) (Biotin)

Cat. Num. AAA6327405

• Gene Names: NXPH1; NPH1; Nbla00697
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NXPH1; Polyclonal Antibody; NT (NXPH1; NPH1; Neurexophilin-1) (Biotin); Neurexophilin-1; anti-NXPH1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-NXPH1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NXPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63~93 amino acids from the N-terminal region of human NXPH1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NXPH1 Antibody (N-term) western blot analysis in mouse cerebellum,brain tissue lysates (35ug/lane).This demonstrates the NXPH1 antibody detected the NXPH1 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with NXPH1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NXPH1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NXPH1 antibody

NXPH1 is a secreted protein with a variable N-terminal domain, a highly conserved, N-glycosylated central domain, a short linker region, and a cysteine-rich C-terminal domain. This protein forms a very tight complex with alpha neurexins, a group of proteins that promote adhesion between dendrites and axons.

Product Categories/Family for anti-NXPH1 antibody

Antibodies; Proteins

References

Xu, J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(5):2136-2140(2010). Shen, L., et al. Neuroimage (2010). In press: Van den Oord, E.J., et al. Arch. Gen. Psychiatry 65(9):1062-1071(2008). Melzer, D., et al. PLoS Genet. 4 (5), E1000072 (2008). Ohira, M., et al. Cancer Lett. 197 (1-2), 63-68 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 23097338
• NCBI GeneID: 30010
• NCBI Accession #: NP_689958.1
• NCBI GenBank Nucleotide #: NM_152745.2
• UniProt Accession #: P58417; Q8NB31
• Molecular Weight: 31,082 Da
• NCBI Official Full Name: neurexophilin-1
• NCBI Official Synonym Full Names: neurexophilin 1
• NCBI Official Symbol: NXPH1
• NCBI Official Synonym Symbols: NPH1; Nbla00697
• NCBI Protein Information: neurexophilin-1
• UniProt Protein Name: Neurexophilin-1
• Protein Family: Neurexophilin
• UniProt Gene Name: NXPH1
• UniProt Synonym Gene Names: NPH1
• UniProt Entry Name: NXPH1_HUMAN

NCBI Description

This gene is a member of the neurexophilin family and encodes a secreted protein with a variable N-terminal domain, a highly conserved, N-glycosylated central domain, a short linker region, and a cysteine-rich C-terminal domain. This protein forms a very tight complex with alpha neurexins, a group of proteins that promote adhesion between dendrites and axons. [provided by RefSeq, Jul 2008]

Uniprot Description

NXPH1: May be signaling molecules that resemble neuropeptides and that act by binding to alpha-neurexins and possibly other receptors (Potential). Belongs to the neurexophilin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: extracellular region

Molecular Function: receptor binding