Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Signal-regulatory protein beta-2 (SIRPB2) Standard Curve.)
Human Signal-Regulatory Protein beta-2 (SIRPB2) ELISA Kit | SIRPB2 elisa kit
Human Signal-Regulatory Protein beta-2 (SIRPB2) ELISA Kit
Gene Names
SIRPB2; PTPN1L; PTPNS1L3; dJ776F14.2
Reactivity
Human
Synonyms
Signal-Regulatory Protein beta-2 (SIRPB2); Human Signal-Regulatory Protein beta-2 (SIRPB2) ELISA Kit; SIRPB2; RP4-776F14.1; PTPN1L; PTPNS1L3; dJ776F14.2; protein tyrosine phosphatase; non-receptor type 1-like; non-receptor type substrate 1-like 3; SIRPB2 elisa kit
Reactivity
Human
Specificity
Human Signal-regulatory protein beta-2 (SIRPB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIRPB2. No significant cross-reactivity or interference between Human SIRPB2 and analogues was observed.
Sequence Length
244
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Signal-regulatory protein beta-2 (SIRPB2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Signal-regulatory protein beta-2 (SIRPB2) Standard Curve.)
Related Product Information for SIRPB2 elisa kit
Description: This Human Signal-regulatory protein beta-2 (SIRPB2) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRPB2 in samples. An antibody specific for SIRPB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIRPB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRPB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRPB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Signal-regulatory protein gamma is a protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described. The 354-amino acid protein shares approximately 75% sequence identity with SIRPB1. It possesses a putative signal sequence, a long extracellular domain, a transmembrane region, a short intracellular domain, and no catalytic domain. RT-PCR analysis detected expression of SIRPG at varying levels in all tissues tested.
Background: Signal-regulatory protein gamma is a protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described. The 354-amino acid protein shares approximately 75% sequence identity with SIRPB1. It possesses a putative signal sequence, a long extracellular domain, a transmembrane region, a short intracellular domain, and no catalytic domain. RT-PCR analysis detected expression of SIRPG at varying levels in all tissues tested.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
signal-regulatory protein beta-2 isoform 2
NCBI Official Synonym Full Names
signal regulatory protein beta 2
NCBI Official Symbol
SIRPB2
NCBI Official Synonym Symbols
PTPN1L; PTPNS1L3; dJ776F14.2
NCBI Protein Information
signal-regulatory protein beta-2
UniProt Protein Name
Signal-regulatory protein beta-2
Protein Family
UniProt Gene Name
SIRPB2
UniProt Synonym Gene Names
PTPN1L; PTPNS1L3; SIRP-beta-2
UniProt Entry Name
SIRB2_HUMAN
Uniprot Description
SIRPB2: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 20p13
Cellular Component: integral to membrane
Research Articles on SIRPB2
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Product Notes
The Human SIRPB2 sirpb2 (Catalog #AAA9714320) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714320 ELISA Kit recognizes Human SIRPB2. It is sometimes possible for the material contained within the vial of "Signal-Regulatory Protein beta-2 (SIRPB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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