PYGL recombinant protein

PYGL, 1-847aa, Human, His tag, E Coli

Cat. Num. AAA205841

• Gene Names: PYGL; GSD6
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: PYGL; 1-847aa; Human; His tag; E Coli; Glycogen phosphorylase liver form isoform 1; Glycogen phosphorylase; liver form isoform 1; GSD6; PYGL recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 30% glycerol, 1mM DTT.
• Concentration: 0.25mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFELRRQ ASMAKPLTDQ EKRRQISIRG IVGVENVAEL KKSFNRHLHF TLVKDRNVAT TRDYYFALAH TVRDHLVGRW IRTQQHYYDK CPKRVYYLSL EFYMGRTLQN TMINLGLQNA CDEAIYQLGL DIEELEEIEE DAGLGNGGLG RLAACFLDSM ATLGLAAYGY GIRYEYGIFN QKIRDGWQVE EADDWLRYGN PWEKSRPEFM LPVHFYGKVE HTNTGTKWID TQVVLALPYD TPVPGYMNNT VNTMRLWSAR APNDFNLRDF NVGDYIQAVL DRNLAENISR VLYPNDNFFE GKELRLKQEY FVVAATLQDI IRRFKASKFG STRGAGTVFD AFPDQVAIQL NDTHPALAIP ELMRIFVDIE KLPWSKAWEL TQKTFAYTNH TVLPEALERW PVDLVEKLLP RHLEIIYEIN QKHLDRIVAL FPKDVDRLRR MSLIEEEGSK RINMAHLCIV GSHAVNGVAK IHSDIVKTKV FKDFSELEPD KFQNKTNGIT PRRWLLLCNP GLAELIAEKI GEDYVKDLSQ LTKLHSFLGD DVFLRELAKV KQENKLKFSQ FLETEYKVKI NPSSMFDVQV KRIHEYKRQL LNCLHVITMY NRIKKDPKKL FVPRTVIIGG KAAPGYHMAK MIIKLITSVA DVVNNDPMVG SKLKVIFLEN YRVSLAEKVI PATDLSEQIS TAGTEASGTG NMKFMLNGAL TIGTMDGANV EMAEEAGEEN LFIFGMRIDD VAALDKKGYE AKEYYEALPE LKLVIDQIDN GFFSPKQPDL FKDIINMLFY HDRFKVFADY EAYVKCQDKV SQLYMNPKAW NTMVLKNIAA SGKFSSDRTI KEYAQNIWNV EPSDLKISLS NESNKVNGN
• Sequence Length: 847

• Applicable Applications for PYGL recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PYGL recombinant protein

PYGL also known as Glycogen phosphorylase, switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. Recombinant human PYGL, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PYGL recombinant protein

Cancer

References

Tomihira M. et al. (2004). Diabetes Res Clin Pract. 65:175-182

NCBI and Uniprot Product Information

• NCBI GI #: 71037379
• NCBI GeneID: 5836
• NCBI Accession #: NP_002854
• NCBI GenBank Nucleotide #: NM_002863.4
• UniProt Accession #: P06737; O60567; O60752; O60913; Q501V9; Q641R5; Q96G82; A6NDQ4; B4DUB7; F5H816
• Molecular Weight: 100.7kDa (879aa)
• NCBI Official Full Name: glycogen phosphorylase, liver form isoform 1
• NCBI Official Synonym Full Names: glycogen phosphorylase L
• NCBI Official Symbol: PYGL
• NCBI Official Synonym Symbols: GSD6
• NCBI Protein Information: glycogen phosphorylase, liver form
• UniProt Protein Name: Glycogen phosphorylase, liver form
• Protein Family: Glycogen phosphorylase
• UniProt Gene Name: PYGL

NCBI Description

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011]

Uniprot Description

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.