rabbit Jumonji Domain Containing Protein 1B Polyclonal Antibody | anti-KDM3B antibody

Jumonji Domain Containing Protein 1B (JMJD1B, JmjC Domain-containing Histone Demethylation Protein 2B, JHDM2B, KDM3B, 5qNCA, Nuclear Protein 5qNCA)

Cat. Num. AAA618004

• Gene Names: KDM3B; 5qNCA; NET22; C5orf7; JMJD1B; KIAA1082
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified by Immunoaffinity chromatography.
• Synonyms: Jumonji Domain Containing Protein 1B; Polyclonal Antibody; Jumonji Domain Containing Protein 1B (JMJD1B; JmjC Domain-containing Histone Demethylation Protein 2B; JHDM2B; KDM3B; 5qNCA; Nuclear Protein 5qNCA); Anti -Jumonji Domain Containing Protein 1B (JMJD1B; anti-KDM3B antibody

• Host: rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of JMJD1B protein.
• Purity/Purification: Affinity Purified; Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-KDM3B antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation.; Dilution: Immunofluorescence: 1:100; Western Blot: 1:1000; Immunoprecipitation: 1:25
• Immunogen: Synthetic peptide (KLH- coupled) corresponding to the human JMJD1B protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of whole cell lysates from HeLa, RAW and COS cells using MBS618004.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HeLa cells using MBS618004 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).)

Related Product Information for anti-KDM3B antibody

The methylation state of lysine residues in histone proteins is major determinant for formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and -ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be di- vided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JH- DM2A, JMJD1B/JHDM2B, JMJD1C/JHDM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen receptor and thyroid receptor and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8).

Product Categories/Family for anti-KDM3B antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 119582546
• NCBI GeneID: 51780
• UniProt Accession #: Q7LBC6; Q9BVH6; Q9BW93; Q9BZ52; Q9NYF4; Q9UPS0; A6H8X7
• Molecular Weight: 191,581 Da
• NCBI Official Full Name: jumonji domain containing 1B, isoform CRA_b
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 3B
• NCBI Official Symbol: KDM3B
• NCBI Official Synonym Symbols: 5qNCA; NET22; C5orf7; JMJD1B; KIAA1082
• NCBI Protein Information: lysine-specific demethylase 3B; OTTHUMP00000224009; nuclear protein 5qNCA; jumonji domain containing 1B; jumonji domain-containing protein 1B; jmjC domain-containing histone demethylation protein 2B
• UniProt Protein Name: Lysine-specific demethylase 3B
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM3B
• UniProt Synonym Gene Names: C5orf7; JHDM2B; JMJD1B; KIAA1082
• UniProt Entry Name: KDM3B_HUMAN

Uniprot Description

JMJD1B: a ubiquitous protein demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity. Three alternatively spliced isoforms have been reported.

Protein type: Oxidoreductase; EC 1.14.11.-; Demethylase

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm

Molecular Function: dioxygenase activity; metal ion binding

Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin modification