Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Semaphorin-5A (SEMA5A) Standard Curve.)
Human Semaphorin-5A (SEMA5A) ELISA Kit | SEMA5A elisa kit
Human Semaphorin-5A (SEMA5A) ELISA Kit
Gene Names
SEMA5A; semF; SEMAF
Reactivity
Human
Synonyms
Semaphorin-5A (SEMA5A); Human Semaphorin-5A (SEMA5A) ELISA Kit; SEMA5A; FLJ12815; SEMAF; semF; sema domain; seven thrombospondin repeats (type 1 and type 1-like); transmembrane domain (TM) and short cytoplasmic domain; 5A; semaphorin 5A; semaphorin F; SEMA5A elisa kit
Reactivity
Human
Specificity
Human Semaphorin-5A (SEMA5A) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMA5A. No significant cross-reactivity or interference between Human SEMA5A and analogues was observed.
Sequence Length
1074
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Semaphorin-5A (SEMA5A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Semaphorin-5A (SEMA5A) Standard Curve.)
Related Product Information for SEMA5A elisa kit
Description: This Human Semaphorin-5A (SEMA5A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA5A in samples. An antibody specific for SEMA5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA5A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA5A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SEMA5A belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene. The deduced 1,077-amino acid protein contains an N-terminal signal sequence, followed by a semaphorin domain, 7 thrombospondin type-1 repeats, a putative transmembrane domain, and a short cytoplasmic tail. It also has 13 conserved cysteines and a conserved N-glycosylation site. Northern blot analysis of whole mouse embryos detected 2 Semf transcripts at embryonic day 10, and their expression decreased toward birth.
Background: SEMA5A belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene. The deduced 1,077-amino acid protein contains an N-terminal signal sequence, followed by a semaphorin domain, 7 thrombospondin type-1 repeats, a putative transmembrane domain, and a short cytoplasmic tail. It also has 13 conserved cysteines and a conserved N-glycosylation site. Northern blot analysis of whole mouse embryos detected 2 Semf transcripts at embryonic day 10, and their expression decreased toward birth.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
semaphorin-5A
NCBI Official Synonym Full Names
semaphorin 5A
NCBI Official Symbol
SEMA5A
NCBI Official Synonym Symbols
semF; SEMAF
NCBI Protein Information
semaphorin-5A
UniProt Protein Name
Semaphorin-5A
Protein Family
UniProt Gene Name
SEMA5A
UniProt Synonym Gene Names
SEMAF; Sema F
NCBI Description
This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]
Uniprot Description
Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
Research Articles on SEMA5A
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Product Notes
The Human SEMA5A sema5a (Catalog #AAA9715994) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715994 ELISA Kit recognizes Human SEMA5A. It is sometimes possible for the material contained within the vial of "Semaphorin-5A (SEMA5A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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