Human Semaphorin 5A (SEMA5A) RTU ELISA Kit | SEMA5A rtu elisa kit

Human Semaphorin 5A (SEMA5A) ELISA Kit

Cat. Num. AAA4502436

• Gene Names: SEMA5A; semF; SEMAF
• Reactivity: Human
• Synonyms: Semaphorin 5A (SEMA5A); Human Semaphorin 5A (SEMA5A) ELISA Kit; SEMAF; SemF; Semaphorin-F; Sema Domain; Immunoglobulin Domain(Ig); Transmembrane Domain(TM)and Short Cytoplasmic Domain 5A; SEMA5A rtu elisa kit

• Reactivity: Human
• Sequence Length: 1074

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.27ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA5A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEMA5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SEMA5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SEMA5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 147904700
• NCBI GeneID: 9037
• NCBI Accession #: NP_003957.2
• NCBI GenBank Nucleotide #: NM_003966.2
• UniProt Accession #: Q13591; O60408; Q1RLL9; D3DTC6
• Molecular Weight: 120,615 Da
• NCBI Official Full Name: semaphorin-5A
• NCBI Official Synonym Full Names: semaphorin 5A
• NCBI Official Symbol: SEMA5A
• NCBI Official Synonym Symbols: semF; SEMAF
• NCBI Protein Information: semaphorin-5A
• UniProt Protein Name: Semaphorin-5A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA5A
• UniProt Synonym Gene Names: SEMAF; Sema F

NCBI Description

This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]

Uniprot Description

Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.