Human Semaphorin 5A (SEMA5A) ELISA Kit | SEMA5A elisa kit

Human Semaphorin 5A (SEMA5A) ELISA Kit

Cat. Num. AAA2019755

• Gene Names: SEMA5A; semF; SEMAF
• Reactivity: Human
• Synonyms: Semaphorin 5A (SEMA5A); Human Semaphorin 5A (SEMA5A) ELISA Kit; SEMAF; SemF; Semaphorin-F; Sema Domain; Immunoglobulin Domain(Ig); Transmembrane Domain(TM)and Short Cytoplasmic Domain 5A; SEMA5A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 5A (SEMA5A).; No significant cross-reactivity or interference between Semaphorin 5A (SEMA5A) and analogues was observed.
• Sequence Length: 1074

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.31ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 5A (SEMA5A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 5A (SEMA5A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA5A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 5A (SEMA5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 5A (SEMA5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 5A (SEMA5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 5A (SEMA5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SEMA5A elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 147904700
• NCBI GeneID: 9037
• NCBI Accession #: NP_003957.2
• NCBI GenBank Nucleotide #: NM_003966.2
• UniProt Accession #: Q13591; O60408; Q1RLL9; D3DTC6
• Molecular Weight: 120,615 Da
• NCBI Official Full Name: semaphorin-5A
• NCBI Official Synonym Full Names: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
• NCBI Official Symbol: SEMA5A
• NCBI Official Synonym Symbols: semF; SEMAF
• NCBI Protein Information: semaphorin-5A
• UniProt Protein Name: Semaphorin-5A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA5A
• UniProt Synonym Gene Names: SEMAF; Sema F
• UniProt Entry Name: SEM5A_HUMAN

NCBI Description

This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]

Uniprot Description

SEMA5A: a single-pass type I membrane protein that may act as positive axonal guidance cues.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: syndecan binding; heparan sulfate proteoglycan binding; axon guidance receptor activity; semaphorin receptor binding

Biological Process: nervous system development; axon guidance; positive regulation of actin filament depolymerization; axonal fasciculation; negative regulation of cell adhesion; negative regulation of axon extension involved in axon guidance; diencephalon development; patterning of blood vessels; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; blood vessel endothelial cell proliferation during sprouting angiogenesis; positive chemotaxis; cell-cell signaling; positive regulation of axon extension involved in axon guidance; positive regulation of endothelial cell proliferation; cell adhesion