Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plasma kallikrein (KLKB1) Standard Curve.)
Human Plasma Kallikrein (KLKB1) ELISA Kit | KLKB1 elisa kit
Human Plasma Kallikrein (KLKB1) ELISA Kit
Gene Names
KLKB1; PKK; PPK; KLK3; PKKD
Reactivity
Human
Synonyms
Plasma Kallikrein (KLKB1); Human Plasma Kallikrein (KLKB1) ELISA Kit; KLKB1; KLK3; PPK; Fletcher factor; kininogenin; plasma kallikrein B1; plasma kallikrein heavy chain; plasma kallikrein light chain; KLKB1 elisa kit
Reactivity
Human
Specificity
Human Plasma kallikrein (KLKB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLKB1. No significant cross-reactivity or interference between Human KLKB1 and analogues was observed.
Sequence Length
436
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plasma kallikrein (KLKB1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plasma kallikrein (KLKB1) Standard Curve.)
Related Product Information for KLKB1 elisa kit
Description: This Human Plasma kallikrein (KLKB1) ELISA Kit employs a two-site sandwich ELISA to quantitate KLKB1 in samples. An antibody specific for KLKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLKB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLKB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLKB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Human prostate-specific antigen (APS) is a kallikrein-like protease present in seminal plasma. It is a single-chain glycoprotein with a molecular mass of about 33 kD that may function normally in the liquefaction of seminal coagulum. Radioimmunoassay of serum levels of this antigen (called PSA in the clinical setting) is useful in the identification and monitoring of prostatic carcinoma. The full-length KLK3 protein contains 261 amino acids. It has a putative signal peptide, followed by a short activating peptide and the protease domain, which includes the catalytic triad of his65, asp120, and ser213. RT-PCR of 35 adult and fetal tissues detected highest expression in prostate. Expression was weak in colon, mammary gland, and parotid, and little to no expression was detected in other tissues examined.
Background: Human prostate-specific antigen (APS) is a kallikrein-like protease present in seminal plasma. It is a single-chain glycoprotein with a molecular mass of about 33 kD that may function normally in the liquefaction of seminal coagulum. Radioimmunoassay of serum levels of this antigen (called PSA in the clinical setting) is useful in the identification and monitoring of prostatic carcinoma. The full-length KLK3 protein contains 261 amino acids. It has a putative signal peptide, followed by a short activating peptide and the protease domain, which includes the catalytic triad of his65, asp120, and ser213. RT-PCR of 35 adult and fetal tissues detected highest expression in prostate. Expression was weak in colon, mammary gland, and parotid, and little to no expression was detected in other tissues examined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
plasma kallikrein isoform 3
NCBI Official Synonym Full Names
kallikrein B1
NCBI Official Symbol
KLKB1
NCBI Official Synonym Symbols
PKK; PPK; KLK3; PKKD
NCBI Protein Information
plasma kallikrein
Protein Family
NCBI Description
This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
Research Articles on KLKB1
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Product Notes
The Human KLKB1 (Catalog #AAA9715555) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715555 ELISA Kit recognizes Human KLKB1. It is sometimes possible for the material contained within the vial of "Plasma Kallikrein (KLKB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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