Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kallikrein 1 (KLK1) Standard Curve.)
Human Kallikrein 1 (KLK1) ELISA Kit | KLK1 elisa kit
Human Kallikrein 1 (KLK1) ELISA Kit
Gene Names
KLK1; hK1; KLKR; Klk6
Reactivity
Human
Synonyms
Kallikrein 1 (KLK1); Human Kallikrein 1 (KLK1) ELISA Kit; KLK1; KLKR; Klk6; hK1; glandular kallikrein 1; kallikrein 1; renal/pancreas/salivary; kallikrein serine protease 1; tissue kallikrein; KLK1 elisa kit
Reactivity
Human
Specificity
Human Kallikrein 1 (KLK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLK1. No significant cross-reactivity or interference between Human KLK1 and analogues was observed.
Sequence Length
245
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kallikrein 1 (KLK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kallikrein 1 (KLK1) Standard Curve.)
Related Product Information for KLK1 elisa kit
Description: This Human Kallikrein 1 (KLK1) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK1 in samples. An antibody specific for KLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: KLK1, or tissue kallikrein is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. Kallikrein 1 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. the deduced 262-amino acid KLK1 protein contains a putative signal peptide, followed by a short activating peptide and the protease domain. The protease domain contains the catalytic triad of his65, asp120, and ser214. RT-PCR detected variable expression of KLK1 in most of the 35 tissues examined. Highest expression was in pancreas, salivary gland, thyroid, parotid gland, fetal and adult skin, kidney, and liver.
Background: KLK1, or tissue kallikrein is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. Kallikrein 1 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. the deduced 262-amino acid KLK1 protein contains a putative signal peptide, followed by a short activating peptide and the protease domain. The protease domain contains the catalytic triad of his65, asp120, and ser214. RT-PCR detected variable expression of KLK1 in most of the 35 tissues examined. Highest expression was in pancreas, salivary gland, thyroid, parotid gland, fetal and adult skin, kidney, and liver.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
kallikrein, partial
NCBI Official Synonym Full Names
kallikrein 1
NCBI Official Symbol
KLK1
NCBI Official Synonym Symbols
hK1; KLKR; Klk6
NCBI Protein Information
kallikrein-1
UniProt Protein Name
Kallikrein-1
Protein Family
UniProt Gene Name
KLK1
UniProt Entry Name
KLK1_HUMAN
NCBI Description
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. [provided by RefSeq, Jul 2008]
Uniprot Description
KLK1: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.21.35; Protease
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleus
Molecular Function: serine-type endopeptidase activity
Biological Process: proteolysis
Disease: Kallikrein, Decreased Urinary Activity Of
Research Articles on KLK1
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Product Notes
The Human KLK1 klk1 (Catalog #AAA9715567) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715567 ELISA Kit recognizes Human KLK1. It is sometimes possible for the material contained within the vial of "Kallikrein 1 (KLK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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