KLKB1 elisa kit

Mouse KLKB1 ELISA Kit

Cat. Num. AAA1752885

• Gene Names: KLKB1; PKK; PPK; KLK3; PKKD
• Reactivity: Mouse; There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: KLKB1; Mouse KLKB1 ELISA Kit; Mouse KLKB1; Plasma kallikrein; Fletcher factor; Kininogenin; Plasma prekallikrein; PKK; Plasma kallikrein heavy chain; Plasma kallikrein light chain; KLK3; KLKB1 elisa kit

• Reactivity: Mouse; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Mouse KLKB1
• Sequence Length: 638

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLKB1 elisa kit

Principle of the Assay: The Picokine™ Mouse KLKB1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse KLKB1 with a 96-well strip plate that is pre-coated with antibody specific for KLKB1. The detection antibody is a biotinylated antibody specific for KLKB1. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated monoclonal antibody from goat. The kit contains recombinant Mouse KLKB1 with immunogen: Expression system for standard: NSO, Immunogen sequence: G20-A638. The kit is analytically validated with ready to use reagents. To measure Mouse KLKB1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse KLKB1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse KLKB1 in the sample.!!Background/Introduction: Plasma kallikrein is a protein that in humans is encoded by the KLKB1 gene. It is mapped to 4q35.2. This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 78191798
• NCBI GeneID: 3818
• NCBI Accession #: NP_000883.2
• NCBI GenBank Nucleotide #: NP_000883.2
• UniProt Accession #: P03952
• NCBI Official Full Name: plasma kallikrein isoform 1 preproprotein
• NCBI Official Synonym Full Names: kallikrein B1
• NCBI Official Symbol: KLKB1
• NCBI Official Synonym Symbols: PKK; PPK; KLK3; PKKD
• NCBI Protein Information: plasma kallikrein
• UniProt Protein Name: Plasma kallikrein
• Protein Family: Plasma kallikrein
• UniProt Gene Name: KLKB1
• UniProt Synonym Gene Names: KLK3
• UniProt Entry Name: KLKB1_HUMAN

NCBI Description

This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily.

Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.34

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; fibrinolysis; extracellular matrix organization and biogenesis; positive regulation of fibrinolysis; zymogen activation; blood coagulation; proteolysis; blood coagulation, intrinsic pathway; Factor XII activation; plasminogen activation

Disease: Prekallikrein Deficiency