Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kallikrein-9 (KLK9) Standard Curve.)
Human Kallikrein-9 (KLK9) ELISA Kit | KLK9 elisa kit
Human Kallikrein-9 (KLK9) ELISA Kit
Gene Names
KLK9; KLKL3; KLK-L3
Reactivity
Human
Synonyms
Kallikrein-9 (KLK9); Human Kallikrein-9 (KLK9) ELISA Kit; KLK9; KLK-L3; KLKL3; kallikrein 9; kallikrein-like protein 3; KLK9 elisa kit
Reactivity
Human
Specificity
Human Kallikrein-9 (KLK9) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLK9. No significant cross-reactivity or interference between Human KLK9 and analogues was observed.
Sequence Length
250
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kallikrein-9 (KLK9) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kallikrein-9 (KLK9) Standard Curve.)
Related Product Information for KLK9 elisa kit
Description: This Human Kallikrein-9 (KLK9) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK9 in samples. An antibody specific for KLK9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLK9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: KLK9 belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues.KLK9 is primarily expressed in thymus, testis, spinal cord, cerebellum, trachea, mammary gland, prostate, brain, salivary gland, ovary, and skin.KLK9 is under steroid hormone regulation in ovarian and breast cancer cell lines and is a potential prognostic marker for early-stage ovarian and breast cancer patients.Based on homology between the human and mouse kallikrein loci, Yousef and Diamandis defined a 300-kb human kallikrein gene region on chromosome 19q13.3-q13.4. Within this region, they identified a new kallikrein gene, KLK9, which they designated KLKL3. The deduced 250-amino acid KLK9 protein has a predicted molecular mass of 27.5 kD and shares 40% amino acid sequence identity with KLK11.
Background: KLK9 belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues.KLK9 is primarily expressed in thymus, testis, spinal cord, cerebellum, trachea, mammary gland, prostate, brain, salivary gland, ovary, and skin.KLK9 is under steroid hormone regulation in ovarian and breast cancer cell lines and is a potential prognostic marker for early-stage ovarian and breast cancer patients.Based on homology between the human and mouse kallikrein loci, Yousef and Diamandis defined a 300-kb human kallikrein gene region on chromosome 19q13.3-q13.4. Within this region, they identified a new kallikrein gene, KLK9, which they designated KLKL3. The deduced 250-amino acid KLK9 protein has a predicted molecular mass of 27.5 kD and shares 40% amino acid sequence identity with KLK11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Kallikrein-9
NCBI Official Synonym Full Names
kallikrein related peptidase 9
NCBI Official Symbol
KLK9
NCBI Official Synonym Symbols
KLKL3; KLK-L3
NCBI Protein Information
kallikrein-9
UniProt Protein Name
Kallikrein-9
Protein Family
UniProt Gene Name
KLK9
UniProt Synonym Gene Names
KLK-L3
UniProt Entry Name
KLK9_HUMAN
NCBI Description
The protein encoded by this gene is a kallikrein-related serine protease. This gene is activated by steroid hormones in a human breast cancer cell line, making it a good marker for cancer detection. The encoded protein is found primarily in the cytoplasm.[provided by RefSeq, Oct 2010]
Uniprot Description
KLK9: is a kallikrein-related serine protease. This gene is activated by steroid hormones in a human breast cancer cell line, making it a good marker for cancer detection. The encoded protein is found primarily in the cytoplasm.[provided by RefSeq, Oct 2010]
Protein type: Secreted; EC 3.4.21.-; Secreted, signal peptide; Protease
Chromosomal Location of Human Ortholog: 19q13.41
Cellular Component: extracellular region
Molecular Function: serine-type endopeptidase activity
Biological Process: proteolysis
Research Articles on KLK9
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Product Notes
The Human KLK9 klk9 (Catalog #AAA9715556) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715556 ELISA Kit recognizes Human KLK9. It is sometimes possible for the material contained within the vial of "Kallikrein-9 (KLK9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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