Human PDGF-DD ELISA Kit | PDGF-DD elisa kit

Human PDGF-DD PicoKine ELISA Kit

Cat. Num. AAA1751605

• Reactivity: Human
• Synonyms: PDGF-DD; Human PDGF-DD PicoKine ELISA Kit; Human PDGF-DD; PDGF-DD elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human PDGFA

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDGF-DD elisa kit

Principle of the Assay: The Picokine Human PDGFA Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PDGFA with a 96-well strip plate that is pre-coated with antibody specific for PDGFA. The detection antibody is a biotinylated antibody specific for PDGFA. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human PDGFA with immunogen: Expression system for standard: E.coli, Immunogen sequence: S87-T211. The kit is analytically validated with ready to use reagents. To measure Human PDGFA, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human PDGFA in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human PDGFA in the sample!!Background/Introduction: PDGF-AA encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.