Rabbit anti-Human Siglec 10 Polyclonal Antibody | anti-SIGLEC10 antibody

Siglec 10, CT (Siglec 10, SLG2, Sialic acid-binding Ig-like lectin 10, Siglec like protein 2) (Biotin)

Cat. Num. AAA6347573

• Gene Names: SIGLEC10; SLG2; PRO940; SIGLEC-10
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Siglec 10; Polyclonal Antibody; CT (Siglec 10; SLG2; Sialic acid-binding Ig-like lectin 10; Siglec like protein 2) (Biotin); SIGLEC10; Siglec-like protein 2; anti-SIGLEC10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SIGLEC10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SIGLEC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SIGLEC10.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SIGLEC10 Antibody western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SIGLEC10 antibody detected the SIGLEC10 protein (arrow).)

Related Product Information for anti-SIGLEC10 antibody

SIGLEC10 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-or 2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. SIGLEC10 interacts with PTPN6/SHP-1 upon phosphorylation. The protein is expressed by peripheral blood leukocytes (eosinophils, monocytes and a natural killer cell subpopulation). Isoform 5 is found to be the most abundant isoform, found in lymph node, lung, ovary and appendix. Isoform 1 is found at high levels and isoform 2 at lower levels in bone marrow, spleen and spinal chord. Isoform 2 is also found in brain. Isoform 4 is specifically found in natural killer cells. SIGLEC10 contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. Phosphorylation of Tyr-667 is involved in binding to PTPN6. The SIGLEC10 gene belongs to the immunoglobulin superfamily.

Product Categories/Family for anti-SIGLEC10 antibody

Antibodies; Lectins

References

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Kitzig, F., et al., Biochem. Biophys. Res. Commun. 296(2):355-362 (2002). Li, N., et al., J. Biol. Chem. 276(30):28106-28112 (2001). Yousef, G.M., et al., Biochem. Biophys. Res. Commun. 284(4):900-910 (2001). Munday, J., et al., Biochem. J. 355 (Pt 2), 489-497 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 283837876
• NCBI GeneID: 89790
• NCBI Accession #: NP_001164627.1
• NCBI GenBank Nucleotide #: NP_001164627.1
• UniProt Accession #: Q96LC7
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 10 isoform 2
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 10
• NCBI Official Symbol: SIGLEC10
• NCBI Official Synonym Symbols: SLG2; PRO940; SIGLEC-10
• NCBI Protein Information: sialic acid-binding Ig-like lectin 10
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 10
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC10
• UniProt Synonym Gene Names: SLG2; Siglec-10
• UniProt Entry Name: SIG10_HUMAN

NCBI Description

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM, Jul 2002]

Uniprot Description

Siglec-10: a cell surface protein belonging to the immunoglobulin superfamily and the SIGLEC (sialic acid binding Ig-like lectin) family. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha2,3- or 2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Five alternatively spliced isoforms have been described.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: plasma membrane; integral to membrane; extracellular region

Molecular Function: carbohydrate binding

Biological Process: cell adhesion