Human MYBBP1A (Myb-binding protein 1A) ELISA Kit | MYBBP1A elisa kit

Human MYBBP1A (Myb-binding protein 1A) ELISA Kit

Cat. Num. AAA7607176

• Gene Names: MYBBP1A; P160; PAP2; Pol5
• Reactivity: Human
• Synonyms: MYBBP1A (Myb-binding protein 1A); Human MYBBP1A (Myb-binding protein 1A) ELISA Kit; MYBBP1A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MUC5AC. No significant cross-reactivity or interference between MUC5AC and analogues was observed.
• Sequence Length: 1332

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MUC5AC were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MUC5AC were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYBBP1A elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MUC5AC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MUC5AC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MUC5AC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MUC5AC can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 157694494
• NCBI GeneID: 10514
• NCBI Accession #: NP_001099008.1
• NCBI GenBank Nucleotide #: NM_001105538.1
• UniProt Accession #: Q9BQG0; Q86VM3; Q9BW49; Q9P0V5; Q9UF99
• Molecular Weight: 149,367 Da
• NCBI Official Full Name: myb-binding protein 1A isoform 1
• NCBI Official Synonym Full Names: MYB binding protein 1a
• NCBI Official Symbol: MYBBP1A
• NCBI Official Synonym Symbols: P160; PAP2; Pol5
• NCBI Protein Information: myb-binding protein 1A
• UniProt Protein Name: Myb-binding protein 1A
• Protein Family: Myb-binding protein
• UniProt Gene Name: MYBBP1A
• UniProt Synonym Gene Names: P160

NCBI Description

This gene encodes a nucleolar transcriptional regulator that was first identified by its ability to bind specifically to the Myb proto-oncogene protein. The encoded protein is thought to play a role in many cellular processes including response to nucleolar stress, tumor suppression and synthesis of ribosomal DNA. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

May activate or repress transcription via interactions with sequence specific DNA-binding proteins (). Repression may be mediated at least in part by histone deacetylase activity (HDAC activity) (). Acts as a corepressor and in concert with CRY1, represses the transcription of the core circadian clock component PER2 (). Preferentially binds to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter (). Has a role in rRNA biogenesis together with PWP1 (PubMed:29065309).