Human Toll Like Receptor 9 (TLR9) RTU ELISA Kit | TLR9 rtu elisa kit

Human Toll Like Receptor 9 (TLR9) ELISA Kit

Cat. Num. AAA4502948

• Gene Names: TLR9; CD289
• Reactivity: Human
• Synonyms: Toll Like Receptor 9 (TLR9); Human Toll Like Receptor 9 (TLR9) ELISA Kit; CD289; TLR9 rtu elisa kit

• Reactivity: Human
• Sequence Length: 1032

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR9 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TLR9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TLR9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TLR9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TLR9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8394456
• NCBI GeneID: 54106
• NCBI Accession #: NP_059138.1
• NCBI GenBank Nucleotide #: NM_017442.3
• UniProt Accession #: Q9NR96; Q6UVZ2; Q9HD68; Q9HD69; Q9HD70; Q9NYC2; Q9NYC3; B3Y661; D1CS56
• Molecular Weight: 114,237 Da
• NCBI Official Full Name: toll-like receptor 9
• NCBI Official Synonym Full Names: toll like receptor 9
• NCBI Official Symbol: TLR9
• NCBI Official Synonym Symbols: CD289
• NCBI Protein Information: toll-like receptor 9
• UniProt Protein Name: Toll-like receptor 9
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR9

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq, Aug 2017]

Uniprot Description

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:11564765, PubMed:17932028). Controls lymphocyte response to Helicobacter infection (). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (PubMed:23857366).