Rabbit anti-Rat Alpha-2-Macroglobulin (a2M) Polyclonal Antibody | anti-a2M antibody

Polyclonal Antibody to Alpha-2-Macroglobulin (a2M)

Cat. Num. AAA2002599

• Gene Names: A2m; Mam; A2m1; A2mb; A2maa; A2MAC1
• Reactivity: Rat
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Alpha-2-Macroglobulin (a2M); Polyclonal Antibody; Polyclonal Antibody to Alpha-2-Macroglobulin (a2M); anti-a2M antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against a2M. It has been selected for its ability to recognize a2M in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGS- RTVEH PFSVEEFVLP KFEVRVTVPE TITILEEEMN VSVCGIYTYG KPVPGRVTVN ICRKYSNPSN CFGEESVAFC EKLSQQLDGR GCFSQLVKTK SFQLKRQEYE MQLDVHAKIQ EEGTGVEETG KGLTKITRTI TKLSFVNVDS HFRQGIPFVG QVLLVDGRGT PIPYETIFIG ADEANLYINT TTDKHGLARF SINTDDIMGT SLTVRAKYKD SNACYGF
• Sequence Length: 1474

• Applicable Applications for anti-a2M antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant a2M (Arg216~Phe437) expressed in E.coli.
• Cross Reactivity: Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2050544
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:RatTissue))

NCBI and Uniprot Product Information

• NCBI GI #: 158138551
• NCBI GeneID: 24153
• NCBI Accession #: NP_036620.2
• NCBI GenBank Nucleotide #: NM_012488.2
• UniProt Accession #: P06238; Q4FZY3
• Molecular Weight: 163,785 Da
• NCBI Official Full Name: alpha-2-macroglobulin
• NCBI Official Synonym Full Names: alpha-2-macroglobulin
• NCBI Official Symbol: A2m
• NCBI Official Synonym Symbols: Mam; A2m1; A2mb; A2maa; A2MAC1
• NCBI Protein Information: alpha-2-macroglobulin
• UniProt Protein Name: Alpha-2-macroglobulin
• Protein Family: Alpha-2-macroglobulin
• UniProt Gene Name: A2m
• UniProt Synonym Gene Names: A2m1; Alpha-2-M

NCBI Description

plays a role in acute phase response; activated form induces a decrease in N-methyl-D-aspartate (NMDA) mediated calcium signaling [RGD, Feb 2006]

Uniprot Description

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.