Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated serine/threonine-protein kinase 2 (MAST2) Standard Curve.)
Human Microtubule-Associated Serine/Threonine-Protein Kinase 2 (MAST2) ELISA Kit | MAST2 elisa kit
Human Microtubule-Associated Serine/Threonine-Protein Kinase 2 (MAST2) ELISA Kit
Gene Names
MAST2; MTSSK; MAST205
Reactivity
Human
Synonyms
Microtubule-Associated Serine/Threonine-Protein Kinase 2 (MAST2); Human Microtubule-Associated Serine/Threonine-Protein Kinase 2 (MAST2) ELISA Kit; MAST2; FLJ39200; KIAA0807; MAST205; MTSSK; RP4-533D7.1; microtubule associated testis specific serine/threonine protein kinase; MAST2 elisa kit
Reactivity
Human
Specificity
Human Microtubule-associated serine/threonine-protein kinase 2 (MAST2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAST2. No significant cross-reactivity or interference between Human MAST2 and analogues was observed.
Sequence Length
1798
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated serine/threonine-protein kinase 2 (MAST2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated serine/threonine-protein kinase 2 (MAST2) Standard Curve.)
Related Product Information for MAST2 elisa kit
Description: This Human Microtubule-associated serine/threonine-protein kinase 2 (MAST2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAST2 in samples. An antibody specific for MAST2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAST2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAST2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAST2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced 1,265-amino acid protein shares significant similarity with mouse Mast205. RT-PCR ELISA detected variable MAST2 expression in all tissues examined. The deduced 1,734-amino acid protein has a calculated molecular mass of 190.5 kD. It had an apparent molecular mass of 205 kD by SDS-PAGE. Mast205 contains a central serine/threonine kinase catalytic domain, which includes an ATP-binding consensus sequence, and several potential phosphorylation sites. Northern blot analysis of mouse tissues detected Mast205 expression only in testis. Mast205 expression increased during postnatal testicular development coincident with meiosis/spermiogenesis. Immunofluorescence microscopy showed Mast205 associated with mouse spermatid manchette.
Background: The deduced 1,265-amino acid protein shares significant similarity with mouse Mast205. RT-PCR ELISA detected variable MAST2 expression in all tissues examined. The deduced 1,734-amino acid protein has a calculated molecular mass of 190.5 kD. It had an apparent molecular mass of 205 kD by SDS-PAGE. Mast205 contains a central serine/threonine kinase catalytic domain, which includes an ATP-binding consensus sequence, and several potential phosphorylation sites. Northern blot analysis of mouse tissues detected Mast205 expression only in testis. Mast205 expression increased during postnatal testicular development coincident with meiosis/spermiogenesis. Immunofluorescence microscopy showed Mast205 associated with mouse spermatid manchette.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microtubule-associated serine/threonine-protein kinase 2 isoform 1
NCBI Official Synonym Full Names
microtubule associated serine/threonine kinase 2
NCBI Official Symbol
MAST2
NCBI Official Synonym Symbols
MTSSK; MAST205
NCBI Protein Information
microtubule-associated serine/threonine-protein kinase 2
UniProt Protein Name
Microtubule-associated serine/threonine-protein kinase 2
Protein Family
UniProt Gene Name
MAST2
UniProt Synonym Gene Names
KIAA0807; MAST205
UniProt Entry Name
MAST2_HUMAN
Uniprot Description
MAST2: Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; AGC group; MAST family
Chromosomal Location of Human Ortholog: 1p34.1
Cellular Component: microtubule cytoskeleton; cytoplasm; plasma membrane
Molecular Function: protein serine/threonine kinase activity; protein binding; microtubule binding; magnesium ion binding; ATP binding; phosphatase binding
Biological Process: spermatid differentiation; regulation of interleukin-12 biosynthetic process; protein amino acid phosphorylation
Research Articles on MAST2
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Product Notes
The Human MAST2 mast2 (Catalog #AAA9715382) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715382 ELISA Kit recognizes Human MAST2. It is sometimes possible for the material contained within the vial of "Microtubule-Associated Serine/Threonine-Protein Kinase 2 (MAST2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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