Mouse Serine/Threonine-Protein Kinase PLK3 (PLK3) ELISA Kit | PLK3 elisa kit

Mouse Serine/Threonine-Protein Kinase PLK3 (PLK3) ELISA Kit

Cat. Num. AAA9717336

• Gene Names: Plk3; Cnk; Fnk; PRK; PLK-3
• Reactivity: Mouse
• Synonyms: Serine/Threonine-Protein Kinase PLK3 (PLK3); Mouse Serine/Threonine-Protein Kinase PLK3 (PLK3) ELISA Kit; PLK3; RP11-269F19.6; CNK; FNK; PRK; FGF-inducible kinase; cytokine-inducible kinase; polo-like kinase 3; proliferation-related kinase; PLK3 elisa kit

• Reactivity: Mouse
• Specificity: Mouse Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLK3. No significant cross-reactivity or interference between Mouse PLK3 and analogues was observed.
• Sequence Length: 631

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Serine/threonine-protein kinase PLK3 (PLK3) Standard Curve.)

Related Product Information for PLK3 elisa kit

Description: This Mouse Serine/threonine-protein kinase PLK3 (PLK3) ELISA Kit employs a two-site sandwich ELISA to quantitate PLK3 in samples. An antibody specific for PLK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: CNK is a member of the 'polo' family of serine/threonine kinases. Members of this family have been implicated in cell division and are important in regulating the onset of mitosis and M-phase progression.The predicted 607-amino acid protein has an N-terminal catalytic domain, including an ATP-binding site, a central putative nuclear targeting signal, and a presumed C-terminal regulatory domain. The CNK protein shows homology with several other members of the 'polo' family; it is 91% identical to mouse Fnk, 50% identical to human PLK, 48% identical to Drosophila polo, and 38% identical to S. cerevisiae Cdc5. Northern blot analysis detected expression of a 2.5-kb CNK transcript in a limited number of human tissues, with placenta containing a moderate level and ovary, lung, and peripheral blood leukocytes containing low levels.

NCBI and Uniprot Product Information

• NCBI GI #: 13878440
• NCBI GeneID: 12795
• UniProt Accession #: Q60806
• NCBI Official Full Name: Serine/threonine-protein kinase PLK3
• NCBI Official Synonym Full Names: polo like kinase 3
• NCBI Official Symbol: Plk3
• NCBI Official Synonym Symbols: Cnk; Fnk; PRK; PLK-3
• NCBI Protein Information: serine/threonine-protein kinase PLK3
• UniProt Protein Name: Serine/threonine-protein kinase PLK3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Plk3
• UniProt Synonym Gene Names: Cnk; Fnk; PLK-3
• UniProt Entry Name: PLK3_MOUSE

NCBI Description

This gene encodes a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal catalytic domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases have primarily been implicated in cell cycle regulation. In mouse, this protein that has been reported to localize to the nucleolus during interphase but is undetectable during mitosis, following nucleolus dissociation during prophase. The protein relocalizes to the nucleolus just prior to cytokinesis and peak levels are detected during G1 of interphase. This gene has been implicated in regulation of entry into S phase, with RNAi-induced depletion resulting in failure to re-enter the cell cycle. Mice deficient for this gene exhibit increased weight and tumor development at advanced age. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]