Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated serine/threonine-protein kinase 1 (MAST1) Standard Curve.)
Human Microtubule-Associated Serine/Threonine-Protein Kinase 1 (MAST1) ELISA Kit | MAST1 elisa kit
Human Microtubule-Associated Serine/Threonine-Protein Kinase 1 (MAST1) ELISA Kit
Gene Names
MAST1; SAST; MCCCHCM
Reactivity
Human
Synonyms
Microtubule-Associated Serine/Threonine-Protein Kinase 1 (MAST1); Human Microtubule-Associated Serine/Threonine-Protein Kinase 1 (MAST1) ELISA Kit; MAST1; KIAA0973; SAST; SAST170; syntrophin associated serine/threonine kinase; MAST1 elisa kit
Reactivity
Human
Specificity
Human Microtubule-associated serine/threonine-protein kinase 1 (MAST1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAST1. No significant cross-reactivity or interference between Human MAST1 and analogues was observed.
Sequence Length
1570
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated serine/threonine-protein kinase 1 (MAST1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Microtubule-associated serine/threonine-protein kinase 1 (MAST1) Standard Curve.)
Related Product Information for MAST1 elisa kit
Description: This Human Microtubule-associated serine/threonine-protein kinase 1 (MAST1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAST1 in samples. An antibody specific for MAST1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAST1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAST1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAST1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MAST1 shares significant similarity with mouse Mast205 (MAST2). RT-PCR ELISA detected high expression in adult brain, spinal cord, and testis, in fetal brain, and in all specific adult brain regions examined. Expression was moderate in ovary, kidney, and fetal liver, low in pancreas, and very low or undetectable in all other tissues examined. PCR analysis of rat tissues showed that, like Sast170, Sast124 was expressed exclusively in brain. Immunohistochemical analysis detected strong Sast124 staining in neurons of the subventricular zone and granule cells of the olfactory bulb, islands of Calleja, hippocampal dentate gyrus, and cerebellum. Sast124 localized in nuclei of neurons, in glia-like cell bodies in the corpus callosum, and in fiber bundles in the spinal trigeminal and solitary tracts.
Background: MAST1 shares significant similarity with mouse Mast205 (MAST2). RT-PCR ELISA detected high expression in adult brain, spinal cord, and testis, in fetal brain, and in all specific adult brain regions examined. Expression was moderate in ovary, kidney, and fetal liver, low in pancreas, and very low or undetectable in all other tissues examined. PCR analysis of rat tissues showed that, like Sast170, Sast124 was expressed exclusively in brain. Immunohistochemical analysis detected strong Sast124 staining in neurons of the subventricular zone and granule cells of the olfactory bulb, islands of Calleja, hippocampal dentate gyrus, and cerebellum. Sast124 localized in nuclei of neurons, in glia-like cell bodies in the corpus callosum, and in fiber bundles in the spinal trigeminal and solitary tracts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microtubule-associated serine/threonine-protein kinase 1
NCBI Official Synonym Full Names
microtubule associated serine/threonine kinase 1
NCBI Official Symbol
MAST1
NCBI Official Synonym Symbols
SAST; MCCCHCM
NCBI Protein Information
microtubule-associated serine/threonine-protein kinase 1
UniProt Protein Name
Microtubule-associated serine/threonine-protein kinase 1
Protein Family
UniProt Gene Name
MAST1
UniProt Entry Name
MAST1_HUMAN
NCBI Description
This gene is a member of the microtubule-associated serine/threonine kinase (MAST) family. The protein encoded by this gene has an N-terminal serine/threonine kinase domain followed by a postsynaptic density protein-95/discs large/zona occludens-1 (PDZ) domain. In mouse and rat, the orthologous protein associates with the cytoskeleton and can bind both beta-2-syntrophin and neuronal nitric oxide synthase (nNOS) through its PDZ domain. In mouse and rat, this protein also co-localizes with dystrophin- and utrophin-associated protein complexes (DAPC/UAPC) in the vascular endothelium of the central nervous system. [provided by RefSeq, May 2017]
Research Articles on MAST1
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Product Notes
The Human MAST1 mast1 (Catalog #AAA9715383) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715383 ELISA Kit recognizes Human MAST1. It is sometimes possible for the material contained within the vial of "Microtubule-Associated Serine/Threonine-Protein Kinase 1 (MAST1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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