Rabbit MAST2 Polyclonal Antibody | anti-MAST2 antibody

MAST2 Polyclonal Antibody

Cat. Num. AAA3007473

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MAST2; Polyclonal Antibody; MAST2 Polyclonal Antibody; Microtubule-associated serine/threonine-protein kinase 2; KIAA0807; MAST205; anti-MAST2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MAST2 polyclonal antibody detects endogenous levels of MAST2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1798

• Applicable Applications for anti-MAST2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 1205-1249 of Human MAST2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MAST2 polyclonal antibody at 1:500 dillutionLane1:PC3 treated with PMA (60ng/ml, 30min) whole cell lysate (40ug) Lane2:The Testis tissue lysate of Rat (40ug) Lane3:The Testis tissue lysate of Mouse (40ug))

Related Product Information for anti-MAST2 antibody

Syntrophin is an adapter protein that functions to bind certain signaling molecules to the dystrophin-associated protein complex. This complex connects the extracellular matrix to the intracellular cytoskeleton for construction and maintenance of the postsynaptic structures in the neuromuscular junction and the central nervous system. Microtubule-associated serine/threonine-protein kinase 2 (MAST205) is a testis-specific, cytoplasmic protein that functions in a multi-protein complex in the maturation of spermatids. MAST205 is involved in linking the dystrophin/utrophin network with microtubule filaments via Syntrophin. By forming a complex with TRAF6, MAST205 regulates lipopolysaccharide-induced IL-12 synthesis in macrophages. This leads to the inhibition of TRAF6 NF-?B activation. Two isoforms exist for MAST205 due to alternative splicing. Isoform 1 represents the full length protein, while isoform 2 lacks the residues 327-396 and 1091-1113. The N-terminus of MAST205 must be phosphorylated in order for ubiquitination to occur at the same site. This ubiquitination leads to the degradation of MAST205 via proteasome-mediated proteolysis.

NCBI and Uniprot Product Information

• NCBI GI #: 112363080
• NCBI Accession #: NP_055927.2
• NCBI GenBank Nucleotide #: NP_055927.2
• UniProt Accession #: Q6P0Q8
• Molecular Weight: ~ 196kDa
• NCBI Official Full Name: microtubule-associated serine/threonine-protein kinase 2 isoform 1
• UniProt Protein Name: Microtubule-associated serine/threonine-protein kinase 2
• Protein Family: Microtubule-associated serine/threonine-protein kinase
• UniProt Gene Name: MAST2
• UniProt Synonym Gene Names: KIAA0807; MAST205
• UniProt Entry Name: MAST2_HUMAN

Uniprot Description

MAST2: Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; AGC group; MAST family

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: microtubule cytoskeleton; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; microtubule binding; magnesium ion binding; ATP binding; phosphatase binding

Biological Process: spermatid differentiation; regulation of interleukin-12 biosynthetic process; protein amino acid phosphorylation