Mouse MX1 Monoclonal Antibody | anti-MX1 antibody

MX1 antibody

Cat. Num. AAA834739

• Gene Names: MX1; MX; MxA; IFI78; IFI-78K
• Applications: Immunofluorescence, Western Blot
• Purity: MX1 antibody was purified by affinity chromatography.
• Synonyms: MX1; Monoclonal Antibody; MX1 antibody; Monoclonal MX1; Anti-MX1; Myxovirus; IFI-78K; IFI78; MX; MxA; Influenza Virus Resistance 1 Interferon-Inducible Protein P78; MX 1; MX-1; anti-MX1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2G12
• Purity/Purification: MX1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 662

• Applicable Applications for anti-MX1 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 1:100; WB: 1:2000
• Biological Significance: In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. MX1 is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection.
• Cross-Reactivity: Human
• Immunogen: MX1 antibody was raised in mouse using a full length recombinant protein of human MX1 (NP_002453) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant MX1 protein using MX1 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MX1 protein (Right) or empty vector (Left) detected with MX1 antibody)

Related Product Information for anti-MX1 antibody

Mouse monoclonal MX1 antibody

Product Categories/Family for anti-MX1 antibody

Infectious Disease

NCBI and Uniprot Product Information

• NCBI GI #: 222136617
• NCBI GeneID: 4599
• NCBI Accession #: NP_002453
• NCBI GenBank Nucleotide #: NM_002462.3
• UniProt Accession #: P20591; Q86YP5; Q96CI3; B2RDA5; B3KU10; C9IYV7; C9J8D6; C9JN19; C9JN88; C9JUL1; C9JZS6; D3DSI8
• Molecular Weight: 55,661 Da
• NCBI Official Full Name: interferon-induced GTP-binding protein Mx1 isoform a
• NCBI Official Synonym Full Names: MX dynamin-like GTPase 1
• NCBI Official Symbol: MX1
• NCBI Official Synonym Symbols: MX; MxA; IFI78; IFI-78K
• NCBI Protein Information: interferon-induced GTP-binding protein Mx1
• UniProt Protein Name: Interferon-induced GTP-binding protein Mx1
• Protein Family: Interferon-induced GTP-binding protein
• UniProt Gene Name: MX1
• UniProt Synonym Gene Names: IFI-78K
• UniProt Entry Name: MX1_HUMAN

NCBI Description

This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

MX1: Dynamin-like GTPase acting as a cell-autonomous host restriction factor against many viral pathogens. Shows activity against influenza viruses, rhabdovirus VSV, bunyavirus LACV, Thogoto virus, measles virus, Hantaan virus, Coxsackie virus CVB, Rift Valley fever virus RVFV, hepatitis B virus HBV, and Crimean- Congo hemorrhagic fever virus CCHFV. Belongs to the dynamin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Hydrolase

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: negative regulation of viral genome replication; apoptosis; metabolic process; cytokine and chemokine mediated signaling pathway; response to virus; innate immune response; defense response; signal transduction; defense response to virus