Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Vasohibin-1 (VASH1) Standard Curve.)
Human Vasohibin-1 (VASH1) ELISA Kit | VASH1 elisa kit
Human Vasohibin-1 (VASH1) ELISA Kit
Gene Names
VASH1; TTCP 1; KIAA1036
Reactivity
Human
Synonyms
Vasohibin-1 (VASH1); Human Vasohibin-1 (VASH1) ELISA Kit; VASH1; KIAA1036; VASH1 elisa kit
Reactivity
Human
Specificity
Human Vasohibin-1 (VASH1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VASH1. No significant cross-reactivity or interference between Human VASH1 and analogues was observed.
Sequence Length
365
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Vasohibin-1 (VASH1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Vasohibin-1 (VASH1) Standard Curve.)
Related Product Information for VASH1 elisa kit
Description: This Human Vasohibin-1 (VASH1) ELISA Kit employs a two-site sandwich ELISA to quantitate VASH1 in samples. An antibody specific for VASH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VASH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VASH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VASH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Vasohibin protein contains a cluster of basic amino acids in its C terminus and no classic secretion signal sequence. Vasohibin expression was robust between embryonic weeks 6 to 12 in heart, brain, kidney, lung, skeletal muscle, and whole embryo. Immunohistochemical analysis detected vasohibin at the endothelial layer of placenta and brain. The deduced protein contains 365 amino acids. RT-PCR ELISA detected intermediate expression in adult heart, brain, lung, liver, kidney, ovary, and spinal cord, as well as in fetal brain. Lower expression was detected in skeletal muscle, testis, pancreas, and fetal liver, and little to no expression was detected in spleen. All specific adult brain regions examined showed low to moderate expression.
Background: Vasohibin protein contains a cluster of basic amino acids in its C terminus and no classic secretion signal sequence. Vasohibin expression was robust between embryonic weeks 6 to 12 in heart, brain, kidney, lung, skeletal muscle, and whole embryo. Immunohistochemical analysis detected vasohibin at the endothelial layer of placenta and brain. The deduced protein contains 365 amino acids. RT-PCR ELISA detected intermediate expression in adult heart, brain, lung, liver, kidney, ovary, and spinal cord, as well as in fetal brain. Lower expression was detected in skeletal muscle, testis, pancreas, and fetal liver, and little to no expression was detected in spleen. All specific adult brain regions examined showed low to moderate expression.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tubulinyl-Tyr carboxypeptidase 1
NCBI Official Synonym Full Names
vasohibin 1
NCBI Official Symbol
VASH1
NCBI Official Synonym Symbols
TTCP 1; KIAA1036
NCBI Protein Information
tubulinyl-Tyr carboxypeptidase 1; vasohibin-1
UniProt Protein Name
Vasohibin-1
Protein Family
UniProt Gene Name
VASH1
UniProt Synonym Gene Names
KIAA1036; VASH
UniProt Entry Name
VASH1_HUMAN
Uniprot Description
VASH1: Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity. Belongs to the vasohibin family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: extracellular space; endoplasmic reticulum; apical part of cell; cytoplasm
Biological Process: negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; response to wounding; angiogenesis; negative regulation of blood vessel endothelial cell migration; cell cycle arrest
Research Articles on VASH1
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Product Notes
The Human VASH1 vash1 (Catalog #AAA9713741) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713741 ELISA Kit recognizes Human VASH1. It is sometimes possible for the material contained within the vial of "Vasohibin-1 (VASH1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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