Human Microfibrillar Associated Protein 5 (MFAP5) ELISA Kit | MFAP5 elisa kit

Human Microfibrillar Associated Protein 5 (MFAP5) ELISA Kit

Cat. Num. AAA2707686

• Gene Names: MFAP5; AAT9; MP25; MAGP2; MAGP-2; MFAP-5
• Reactivity: Human
• Synonyms: Microfibrillar Associated Protein 5 (MFAP5); Human Microfibrillar Associated Protein 5 (MFAP5) ELISA Kit; MAGP2; MP25; Microfibril-associated glycoprotein 2; MFAP5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Microfibrillar Associated Protein 5 (MFAP5).; No significant cross-reactivity or interference between Microfibrillar Associated Protein 5 (MFAP5) and analogues was observed.
• Sequence Length: 173

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.057ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microfibrillar Associated Protein 5 (MFAP5) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microfibrillar Associated Protein 5 (MFAP5) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MFAP5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microfibrillar Associated Protein 5 (MFAP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microfibrillar Associated Protein 5 (MFAP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microfibrillar Associated Protein 5 (MFAP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microfibrillar Associated Protein 5 (MFAP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505089
• NCBI GeneID: 8076
• NCBI Accession #: NP_003471.1
• NCBI GenBank Nucleotide #: NP_003471.1
• UniProt Accession #: Q13361
• NCBI Official Full Name: microfibrillar-associated protein 5 isoform a
• NCBI Official Synonym Full Names: microfibril associated protein 5
• NCBI Official Symbol: MFAP5
• NCBI Official Synonym Symbols: AAT9; MP25; MAGP2; MAGP-2; MFAP-5
• NCBI Protein Information: microfibrillar-associated protein 5
• UniProt Protein Name: Microfibrillar-associated protein 5
• Protein Family: Microfibrillar-associated protein
• UniProt Gene Name: MFAP5
• UniProt Synonym Gene Names: MAGP2; MFAP-5; MAGP-2
• UniProt Entry Name: MFAP5_HUMAN

NCBI Description

This gene encodes a 25-kD microfibril-associated glycoprotein which is a component of microfibrils of the extracellular matrix. The encoded protein promotes attachment of cells to microfibrils via alpha-V-beta-3 integrin. Deficiency of this gene in mice results in neutropenia. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

Uniprot Description

MFAP5: Component of the elastin-associated microfibrils. Belongs to the MFAP family.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 12p13.1-p12.3

Cellular Component: microfibril; extracellular region

Molecular Function: extracellular matrix structural constituent

Biological Process: extracellular matrix organization and biogenesis; fibril organization and biogenesis

Disease: Aortic Aneurysm, Familial Thoracic 9