MFAP5 recombinant protein

MFAP5 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121208

• Gene Names: MFAP5; MP25; MAGP2
• Purity: >85% as determined by SDS-PAGE
• Synonyms: MFAP5; MFAP5 Protein; Human; Recombinant (His Tag); Human MFAP5/MAGP2 Protein (His Tag); AAT9 Protein; MAGP-2 Protein; MAGP2 Protein; MFAP-5 Protein; MP25 Protein; microfibrillar associated protein 5; MFAP5 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu173

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Arg 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human MFAP5 (Q13361)(Met1-Leu173) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MFAP5 recombinant protein

Background: MFAP5 (Microfibril Associated Protein 5, also known as MAGP2) is a Protein Coding gene. MFAP5 is a component of the elastin-associated microfibrils. It belongs to the MFAP family. As a 25-kD microfibril-associated glycoprotein, MFAP5 is rich in serine and threonine residues. It stimulates the assembly of elastic fibers, a complex structure composed of a tropoelastin inner core and microfibril outer mantle comprising proteins such as fibrillins and microfibril-associated glycoproteins that guide tropoelastin deposition. MFAP5 also stabilizes type 1 procollagen and thus plays an important role in extracellular matrix homeostasis. It has multiple binding regions on fibrillins and has a covalent periodic association with fibrillin-containing microfibrils. Diseases associated with MFAP5 include Aortic Aneurysm, Familial Thoracic 9, and Familial Thoracic Aortic Aneurysm And Aortic Dissection.

References

Hatzinikolas G, et al. (1998) The exon structure of the human MAGP-2 gene. Similarity with the MAGP-1 gene is confined to two exons encoding a cysteine-rich region. J Biol Chem. 273(45):29309-14.Gibson MA, et al. (1996) Further characterization of proteins associated with elastic fiber microfibrils including the molecular cloning of MAGP-2 (MP25). J Biol Chem. 271(2): 1096-103.Miyamoto A, et al. (2006) Microfibrillar proteins MAGP-1 and MAGP-2 induce Notch1 extracellular domain dissociation and receptor activation. J Biol Chem. 281(15):10089-97.

NCBI and Uniprot Product Information

• NCBI GI #: 4505089
• NCBI GeneID: 8076
• NCBI Accession #: NP_003471.1
• NCBI GenBank Nucleotide #: NM_003480.2
• UniProt Accession #: Q13361; B0AZL6; D3DUV1
• Molecular Weight: 19,612 Da
• NCBI Official Full Name: microfibrillar-associated protein 5
• NCBI Official Synonym Full Names: microfibrillar associated protein 5
• NCBI Official Symbol: MFAP5
• NCBI Official Synonym Symbols: MP25; MAGP2
• NCBI Protein Information: microfibrillar-associated protein 5; MAGP-2; MFAP-5; microfibril-associated glycoprotein 2; microfibril-associated glycoprotein-2
• UniProt Protein Name: Microfibrillar-associated protein 5
• Protein Family: Microfibrillar-associated protein
• UniProt Gene Name: MFAP5
• UniProt Synonym Gene Names: MAGP2; MFAP-5; MAGP-2
• UniProt Entry Name: MFAP5_HUMAN

NCBI Description

This gene encodes a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. It lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this encoded protein has some functions that are distinct from those of MFAP2. [provided by RefSeq, Jul 2008]

Uniprot Description

MFAP5: Component of the elastin-associated microfibrils. Belongs to the MFAP family.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 12p13.1-p12.3

Cellular Component: microfibril; extracellular region

Molecular Function: extracellular matrix structural constituent

Biological Process: extracellular matrix organization and biogenesis; fibril organization and biogenesis

Disease: Aortic Aneurysm, Familial Thoracic 9